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γ-Glutamyl transpeptidase demonstrated in tissue sections embedded in glycol methacrylate resin

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Summary

A method is described for the histochemical demonstration of γ-glutamyl transpeptidase in tissue sections embedded in glycol methacrylate at low temperature. Enzyme activity was preserved by a short (3 h) fixation of tissue in 4% paraformaldehyde at 4° C prior to embedding at 4° C. Tissue embedded in glycol methacrylate combined good morphology with accurate enzyme localization. Blocks of the embedded tissue could be stored at room temperature for at least 3 months without loss of enzyme activity. The resin is non-fluorescent, allowing the use of the fluorescent coupling agent 5-nitrosalicylaldehyde to visualize the reaction product.

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References

  • ASHFORD, A. E., ALLAWAY, W. G. & MCCULLY, H. E. (1972) Low temperature embedding in glycol methacrylate for enzyme histochemistry in plant and animal tissues.J. Histochem. Cytochem. 20, 986–90.

    Google Scholar 

  • BECKSTEAD, J. H. & BAINTON, D. F. (1980) Enzyme histochemistry on bone marrow biopsies: Reactions useful in the differential diagnosis of leukaemias and lymphomas applied to 2-micron plastic sections.Blood 55, 386–94.

    Google Scholar 

  • COHEN, M. B., BECKSTEAD, J. H., FERELL, L. D. & YEN, T. S. B. (1986a) Enzyme histochemistry of hepatocellular neoplasms.Amer. J. Surg. Pathol. 10, 789–94.

    Google Scholar 

  • COHEN, M. B., MILLER, T. R. & BECKSTEAD, J. H. (1986b) Enzyme histochemistry and thryoid neoplasia.Amer. J. Clin. Pathol. 85, 668–73.

    Google Scholar 

  • DIERICKX, P. J. (1981) Urinary gamma-glutamyl transferase as an indicator of acute nephrotoxicity in rats.Arch. Toxicol. 47, 209–15.

    Google Scholar 

  • GERRITS, P. O. & ZUIDVELD, R. (1983) The influence of dehydration media and catalyst systems upon the enzyme activity of tissues embedded in 2-hydroxyethyl methacrylate: an evaluation of three dehydration media and two catalyst systems.Mikroskopie 40, 321–28.

    Google Scholar 

  • GOSSRAU, R. (1980) Conventional techniques for membrane-bound enzymes. InTrends in Enzyme Histochemistry and Cytochemistry (edited by EVERED, D. & O'CONNOR, M.), pp. 67–80. (Ciba Foundation Symposium 73). Amsterdam, Oxford & New York: Excerpta Medica.

    Google Scholar 

  • GOSSRAU, R. (1985) Cytochemistry of membrane proteases.Histochem. J. 17, 737–71.

    Google Scholar 

  • GRIFFITH, O. W. & MEISTER, A. (1979) Glutathione: Interorgan translocation, turnover and metabolism.Proc. Natl. Acad. Sci. USA 76, 5606–10.

    Google Scholar 

  • HIGUCHI, S., SUGA, M., DANNENBERG, A. M. & SCHOFIELD, B. H. (1979) Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections.Stain Technol. 54, 5–12.

    Google Scholar 

  • LITWIN, J. A. (1985) Light microscopic histochemistry on plastic sections.Prog. Histochem. Cytochem. 16, 1–84.

    Google Scholar 

  • MEISTER, A. (1984) New aspects of glutathione biochemistry and transport: Selective alteration of glutathione metabolism.Fed. Proc. 43, 3031–42.

    Google Scholar 

  • MEISTER, A. & ANDERSON, M. E. (1983) Glutathione.Ann. Rev. Biochem. 52, 711–60.

    Google Scholar 

  • MEISTER, A., TATE, S. S. & GRIFFITH, O. W. (1981) γ-Glutamyl transpeptidase.Methods Enzymol. 77, 237–53.

    Google Scholar 

  • NAMBA, M., DANNENBERG, A. M. & TANAKA, F. (1983) Improvement in the histochemical demonstration of acid phosphatase, β-galactosidase and non-specific esterase in glycol methacrylate tissue sections by cold temperature embedding.Stain Technol. 58, 207–13.

    Google Scholar 

  • RUTENBURG, A. M., KIM, H., FISCHBEIN, J. W., HAWKER, J. S., WASSERKRUG, H. L. & SELIGMAN, A. M. (1969) Histochemical and ultrastructural demonstration of γ-glutamyl transpeptidase activity.J. Histochem. Cytochem. 17, 517–26.

    Google Scholar 

  • SINGH, J., CHANDER, J., SINGH, S., SINGH, G. & ATAL, C. K. (1986) γ-Glutamyl transpeptidase: a novel biochemical marker in inflammation.Biochem. Pharmacol. 35 3753–60.

    Google Scholar 

  • TATE, S. S., THOMPSON, G. A. & MEISTER, A. (1976) Recent studies on gamma-glutamyl transpeptidase. InGlutathione, metabolism and function (edited by ARIAS, I. M. & JAKOBY, W. B.) pp. 45–55. New York; Raven.

    Google Scholar 

  • TE VELDE, J., BURKHARDT, R., KLEIVERDA, K., LEENHEERS-BINNENDIJK, L. & SOMMERFELD, W. (1977) Methyl methacrylate as an embedding medium in histopathology.Histopathology 1 319–30.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, University Medical Buildings, University of Aberdeen, Foresterhill, AB9 2ZD, Aberdeen, UK

    G. I. Murray & S. W. B. Ewen

  2. Department of Pharmacology, University of Aberdeen, Marischal College, AB9 1AS, Aberdeen, UK

    M. D. Burke

Authors
  1. G. I. Murray
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  2. M. D. Burke
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  3. S. W. B. Ewen
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Murray, G.I., Burke, M.D. & Ewen, S.W.B. γ-Glutamyl transpeptidase demonstrated in tissue sections embedded in glycol methacrylate resin. Histochem J 19, 476–482 (1987). https://doi.org/10.1007/BF01675417

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  • DOI: https://doi.org/10.1007/BF01675417

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