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Phenotypic differentiation patterns of the human monocyte/macrophage system

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Summary

In the present study phenotypic properties of non-stimulated and stimulated blood monocytes and of their normal macrophage derivatives were studied applying enzyme cytochemistry, isoenzyme analysis of acid esterase (EC 3.1.1.6), and immunohistochemical staining using a panel of newly established monoclonal antibodies specific for the monocyte/macrophage lineage. Certain marker profiles could be established for the various normal subpopulations within the monocyte/macrophage system, which were also observable in epithelioid cells and U-937 cell line considered as reactive and neoplastic differentiation variants of monocytes, respectively. Alveolar macrophages, in contrast to the other analysed monocyte/macrophage populations, showed a highly activated phenotype comparable to lymphokine stimulated blood monocytes and epithelioid cells. The results underline the concept that the adaptation of monocytes/macrophages to their particular microenvironment is of decisive importance for their definitive differentiation.

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  1. Institute of Pathology, University of Kiel, Hospitalstrasse 42, 2300, Kiel, West Germany

    Hans Kreipe, Heinz-J. Radzun & Mohammad R. Parwaresch

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  1. Hans Kreipe
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  2. Heinz-J. Radzun
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  3. Mohammad R. Parwaresch
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Kreipe, H., Radzun, HJ. & Parwaresch, M.R. Phenotypic differentiation patterns of the human monocyte/macrophage system. Histochem J 18, 441–450 (1986). https://doi.org/10.1007/BF01675337

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