Summary
A quantitative method for determination of endogenous serum proteins has been established and tested in an experimental model of peritumoural brain oedema in cats. Rocket and crossed immunoelectrophoresis were applied for determination of total serum proteins, albumin, IgG and haemoglobin in blood and brain homogenates. Modifications such as the use of non-ionic detergents and of antisera with different specificity were established for each antigen under investigation. The content of total serum proteins, albumin and IgG was substantially higher in tumour and peritumoural brain tissue than in the non-oedematous brain. The measurement of haemoglobin allowed the calculation of blood volume and, in consequence, the differentiation between intra- and extravascular serum proteins. The results are in line with earlier measurements obtained by different analytical methods and demonstrate that the present technique provides a reliable approach for the quantitative assessment of serum protein extravasation.
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References
Axelsen NH (1973) Quantitative immunoelectrophoretic methods as tools for a polyvalent approach to standardization in the immunochemistry of Candida albicans. Infect Immun 7: 949–960
Baker RN, Ancilla PA, Pollock PS, Frommes SP (1971) The movement of exogenous protein in experimental cerebral edema. An electron microscopic study after freeze-injury. J Neuropathol Exp Neurol 30: 668–679
Bjerrum OJ (1983) Crossed hydrophobic interaction immunoelectrophoresis. Scan J Immunol 18 [Suppl] 10: 303–312
Bjerrum OJ, Bhakdi S (1983) Detergent immunoelectrophoresis of membrane proteins — general principles. Scan J Immunol 17 [Suppl] 10: 289–301
Bjerrum OJ, Lowenstein H (1983) Immunoelectrophoresis utilizing carbamylated antibodies. Scan J Immunol 17 [Suppl] 10: 225–232
Blasberg RG, Gazendam J, Patlak CS, Fenstermacher JD (1980) Quantitative autoradiographic studies of brain edema and a comparison of multi-isotope autoradiographic techniques. Adv Neurol 28: 255–270
Bodsch W, Hürter T, Hossmann K-A (1982) Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of rat brain. Brain Res 249: 111–121
Bothe H-W, Bodsch W, Hossmann K-A (1984) Relationship between specific gravity, water content, and serum protein extravasation in various types of vasogenic brain edema. Acta Neuropathol (Berl) 64: 37–42
Clasen RA, Bezkorovainy A, Pandolfi S (1982) Protein and electrolyte changes in experimental cerebral edema. J Neuropathol Exp Neurol 41: 113–128
Crowle AJ (1973) Immunodiffusion. Second Edition. Academic Press, New York, pp 83–90
Ebhardt G, van den Kerckhoff W, Matsuoka Y, Hossmann K-A (1982) Morphologische Befunde bei experimentell erzeugten Gliomen in Katzen. Neurochirurgia (Stuttg) 25: 177–185
Go KG, Patberg WR, Teelken AW, Gazendam J (1976) The Starling hypothesis of capillary fluid exchange in relation to brain edema. In: Pappius HMet al (eds) Dynamics of brain edema. Springer, Berlin Heidelberg New York, pp 63–76
Grubb AO (1983) Crosses immunoelectrophoresis. Scand J Immunol 17 [Suppl] 10: 113–124
Harboe NMG, Inglid A (1983) Immunization, isolation of immunoglobulins and antibody titre determination. Scand J Immunol 17 [Suppl] 10: 345–351
Helenius A, Simons K (1975) Solubilisation of membranes by detergents. Biochim Biophys ACta 415: 28–79
Hoiby N, Axelsen NH (1983) Crossed immunoelectrophoresis as modified for quantitative purpose. Scand J Immunol 17: 125–134
Hossmann K-A, Wechsler W, Wilmes F (1979) Experimental peritumorous edema. Morphological and pathophysiological observations. Acta Neuropathol (Berl) 45: 195–203
Hossmann K-A, Szymas J, Seo K, Assheuer J, Jrajewski S (1989) Experimental transplantation gliomas in the adult cat brain. 2. Pathophysiology and magnetic resonance imaging. Acta Neurochir (Wien) 98: 149–200
Kampen EJ, Zijlstra WG (1961) Standardization of hemoglobinometry. II. The hemoglobincyanide method. Clin Chim Acta 6: 538–544
Klatzo I (1967) Presidential address: Neuropathological aspects of brain edema. J Neuropathol Exp Neurol 25: 1–14
Klatzko I, Chui E, Fujiwara K, Spatz M (1980) Resolution of vasogenic brain edema. Adv Neurol 28: 359–373
Labeta MO, Fernandez N, Festenstein H (1988) Solubilisation effect of Nonidet P-40, Triton X-100 and CHASP in the detection of MHC-like glycoproteins. J Immunol Meth 112: 133–138
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Marmarou A, Tanaka K, Shulman K (1982) An improved gravimetric measure of cerebral edema. J Neurosurg 56: 246–253
Shapshak P, Tourtellotte WW, Staugaitis S, Cowan T, Ingram T, Weil ML, Bliss D, Tourtellotte WG (1983) Quantitation of human immunoglobulins G and Albumin in electroimmunodiffusion gels containing ionic and nonionic detergents. Anal Biochem 132: 305–311
Szymas J, Hossmann K-A (1984) Effect of dexamethasone on glial fibrillary acidic protein in peritumorous edema in cats: A morphometric study. Acta Neuropathol (Berl) 62: 309–315
Tengvar C, Hultström D, Olsson Y (1983) An improved Percoll density gradient for measurements of experimental brain edema. Acta Neuropathol (Berl) 61: 201–206
Ter-Pogossian MM, Phelps ME, Grubb RL Jr, Gado M (1971) Measure of regional cerebral blood volume in vivo by means of excited fluorescent X-radiation, and factors affecting this parameter. Europ Neurol 6: 218–223
Verbruggen R (1975) Quantitative immunoelectrophoretic methods: a literature survey. Clin Chem 21: 5–43
Wechsler W, Szymas J, Bilzer T, Hossmann K-A (1989) Experimental transplantation gliomas in the adult cat brain. 1. Experimental model and neuropathology. Acta Neurochir (Wien) 98: 77–89
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Szymas, J., Hossmann, K.A. Determination of endogenous serum proteins in normal and oedematous brain tissue of cat by rocket and crossed immunoelectrophoresis. Acta neurochir 105, 169–177 (1990). https://doi.org/10.1007/BF01670002
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DOI: https://doi.org/10.1007/BF01670002