Summary
A 0.75% agarose overlay procedure is described which has the advantages of being easy to prepare and perform, and is applicable to several commonly used insect cell lines and viruses. In addition, plaque variants which do not produce polyhedra can be easily detected.
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Fraser, M.J. Simplified agarose overlay plaque assay for insect cell lines and insect nuclear polyhedrosis viruses. Journal of Tissue Culture Methods 7, 43–46 (1982). https://doi.org/10.1007/BF01666880
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DOI: https://doi.org/10.1007/BF01666880