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Preparation of primary monolayer cultures of mouse pancreatic epithelial cells

  • Published:
Journal of tissue culture methods

Summary

We describe a technique using normal and diabetic (dbdb) mice to establish primary pancreatic cultures that spread and assume a characteristic epithelial morphology. These cultures contain 4 to 7% beta cells, secrete insulin in response to stimuli for 10 to 14 d, contain few fibroblasts, and have a cell viability that is greater than 95%. The cells attach firmly to glass cover slips and are ideal for the study of insulin secretory granules or contractile proteins using indirect immunofluorescence.

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Authors and Affiliations

  1. Departments of Medicine and Cell Biology, Baylor College of Medicine, 1200 Moursund Avenue, 77030, Houston, Texas, USA

    W. E. Bolton, S. P. Terrell, K. L. Andrews & A. E. Boyd III

Authors
  1. W. E. Bolton
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  2. S. P. Terrell
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  3. K. L. Andrews
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  4. A. E. Boyd III
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Bolton, W.E., Terrell, S.P., Andrews, K.L. et al. Preparation of primary monolayer cultures of mouse pancreatic epithelial cells. Journal of Tissue Culture Methods 7, 39–42 (1982). https://doi.org/10.1007/BF01666879

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  • DOI: https://doi.org/10.1007/BF01666879

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