Summary
An effective method is described for the dissociation of ovine thyroid gland tissue using collagenase as the dispersing agent. The procedure results in large yields of cells from relatively small amounts of thyroid tissue. The conditions for preparing primary cultures of the cells which result in optimum morphologic and metabolic responses to thyroid-stimulating hormone are presented.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Pulvertaft, R. J. V.; Davies, J. R.; Weiss, L.; Wilkinson, J. H. Studies on tissue cultures of human pathological thyroids. J. Path. Bact. 77: 19–32; 1959.
Tong, W.; Kerkof, P. R.; Chaikoff, I. L. Iodine metabolism of dispersed thyroid cells obtained by trypsinization of sheep thyroid glands. Biochim. Biophys. Acta 60: 1–19; 1962.
Dickson, J. A. Effects of thyrotrophic hormone on thyroid cells in filter-well culture. Endocrinology 79: 721–731; 1966.
Fayet, G.; Pacheco, H.; Tixier, R. Sur la réassociation in vitro des cellules isolées de thyroide de porc et la synthèse de la TG. I. Conditions pour l'induction des réassociations cellulaires par la thyréostimuline. Bull. Soc. Chim. Biol. 52: 299–306; 1970.
Tong, W. The isolation and culture of thyroid cells. Fleischer, S.; Packer, L., eds. Methods in enzymology. New York: Academic Press, Inc.; Vol. 32. 1974: 745–758.
Speight, J. W.; Baba, W. I.; Wilson, G. M. The effect of propylthiouracil and thyroid-stimulating hormone on the survival of rat thyroid cells in vivo and in vitro. J. Endocr. 41: 577–591; 1968.
El-Khatib, S. M.; Haldar, J.; Starr, J. L. The effect of LATS and TSH on protein and RNA synthesis in isolated thyroid cells. Proc. Soc. Exp. Biol. Med. 143: 869–878; 1973.
Hilfer, S. R. Collagenase treatment of chick heart and thyroid. Kruse, P. F., Jr.; Patterson, M. K., Jr., eds. Tissue culture methods and applications. New York: Academic Press, Inc.; 1973: 16–20.
Rapoport, B. Dog thyroid cells in monolayer tissue culture: Adenosine 3′,5′ -cyclic monophosphate response to thyrotropic hormone. Endocrinology 98: 1189–1197; 1976.
Westermark, B.; Karlson, F. A.; Wålinker, O. Thyrotropin is not a growth factor for human thyroid cells in culture. Proc. Natl. Acad. Sci. USA. 76: 2022–2026; 1979.
Zamora, P. O.; Waterman, R. E.; Kerkof, P. R. Early effects of thyrotropin on the surface morphology of thyroid cells in culture. J. Ultrastruct. Res. 69: 196–210; 1979.
Nilson, J. H.; Orlicky, D. J.; Kerkof, P. R. In vitro effects of thyroid-stimulating hormone on total and polysomal ribonucleic acid synthesis in monolayer cultures of sheep thyroid cells. Endocrinology 107: 262–269; 1980.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kerkof, P.R. Preparation of primary cultures of ovine thyroid gland cells. Journal of Tissue Culture Methods 7, 23–26 (1982). https://doi.org/10.1007/BF01666876
Issue Date:
DOI: https://doi.org/10.1007/BF01666876