Summary
A technique for culturing small quantities of mammalian cells on modified microscope slides is described. The modified microscope slides were Bellco Glass, Inc., toxoplasmosis slides and the cell cultures used were early passage bovine embryonic lung cells and continuous cell lines of porcine and canine origins. The slide cell cultures were either uninfected or infected with selected viruses or the obligate intracellular protozoanEncephalitozoon caniculi for utilization in direct and indirect fluorescent antibody testing or in peroxidase antiperoxidase immunosorbant assays.
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England, J. J.; McChesney, A. E. Laboratory diagnosis of equine adenoviral infections. Proc. 17th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians. 1974: 157–168.
Gardner, P. S.; McQuillin, J. Rapid virus diagnosis application of immuno-fluorescence. London: Butterworth and Co.; 1974.
Gerna, G.; McCloud, C. J.; Chambers, R. W. Immunoperoxidase technique for detection of antibodies to human cytomegalovirus. J. Clin. Microbiol. 3(3): 364–372; 1976.
Kodama, Y.; Ogata, M.; Shimizu, Y. Detection of antibody against transmissible gastroenteritis virus of pigs by indirect immunoperoxidase antibody test. Amer. J. Vet. Res. 41: 133–135; 1980.
Kawamura, A. J., ed. Fluorescent antibody techniques and their applications, 203 pp. Baltimore: University Park Press; 1969.
Yen, C.; Solorzano, R. F.; Morehouse, L. G. Studies on transmissible gastroenteritis virus by immunopathological techniques. Proc. 19th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians. 1976: 21–46.
Post, J. E.; Wellenstein, R. C.; Clarke, R. D. Serologic tests for feline infectious peritonitis using transmissible gastroenteritis virus. Proc. 21st Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians. 1978: 427–436.
Sternberger, L. A. Immunocytochemistry. Sternberger, L. A. ed. Englewood Cliffs, NJ: Prentice-Hall; 1974: 129–171.
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England, J.J., Riegel, C., Jankowski, L.D. et al. A micro cell culture method utilizing modified microscope slides for use in fluorescent antibody and enzyme immunoassays. Journal of Tissue Culture Methods 7, 13–17 (1982). https://doi.org/10.1007/BF01666874
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DOI: https://doi.org/10.1007/BF01666874