, Volume 156, Issue 1–2, pp 45–56 | Cite as

Polysaccharide and protein secretion by grass microhairs

A cytochemical study at light and electron microscopic levels
  • Vindhya Amarasinghe


Secretory activities of bicellular microhairs from grasses belonging to the subfamilies Chloridoideae, Arundinoideae, Panicoideae, and Bambusoideae, and including the “chloridoid”, “panicoid” and “Enneapogon” microhair morphological types, have been investigated. Light microscopic histochemistry indicated that all microhairs studied secrete polysaccharide and protein (or glycoprotein), including those which also secrete salt. Localization of polysaccharide at ultrastructural level using periodic acid-thiocarbohydrazidesilver proteinate staining revealed that in “panicoid type” microhairs dictyosomes are involved in polysaccharide secretion, whereas in the “chloridoid” and “Enneapogon” types “partitioning membranes” seem to be involved instead.


Cytochemistry Grasses Microhairs Partitioning membranes Polysaccharides Ultrastructure 



silver precipitates representing localization of polysaccharide


basal cell




cap cell


cuticular chamber


system of membrane bound channels and vesicles




cell wall








partitioning membranes


rough endoplasmic reticulum


secretory material


starch grain


unstained dictyosome cisternae




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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Vindhya Amarasinghe
    • 1
  1. 1.Research School of Biological SciencesAustralian National UniversityCanberraAustralia

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