Summary
Secretory activities of bicellular microhairs from grasses belonging to the subfamilies Chloridoideae, Arundinoideae, Panicoideae, and Bambusoideae, and including the “chloridoid”, “panicoid” and “Enneapogon” microhair morphological types, have been investigated. Light microscopic histochemistry indicated that all microhairs studied secrete polysaccharide and protein (or glycoprotein), including those which also secrete salt. Localization of polysaccharide at ultrastructural level using periodic acid-thiocarbohydrazidesilver proteinate staining revealed that in “panicoid type” microhairs dictyosomes are involved in polysaccharide secretion, whereas in the “chloridoid” and “Enneapogon” types “partitioning membranes” seem to be involved instead.
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Abbreviations
- Ag:
-
silver precipitates representing localization of polysaccharide
- BC:
-
basal cell
- C:
-
cuticle
- CC:
-
cap cell
- CH:
-
cuticular chamber
- CN:
-
system of membrane bound channels and vesicles
- CP:
-
chloroplast
- CW:
-
cell wall
- D:
-
dictyosomes
- M:
-
mitochondria
- N:
-
nucleus
- PTM:
-
partitioning membranes
- RER:
-
rough endoplasmic reticulum
- S:
-
secretory material
- St:
-
starch grain
- US:
-
unstained dictyosome cisternae
- V:
-
vesicle
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Amarasinghe, V. Polysaccharide and protein secretion by grass microhairs. Protoplasma 156, 45–56 (1990). https://doi.org/10.1007/BF01666505
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DOI: https://doi.org/10.1007/BF01666505