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Three-dimensional analysis of hemopoietic cellular interactions in vitro using gelfoam sponge matrices

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Journal of tissue culture methods

Summary

Methods are described for preparation of medium and explants of long bone (or marrow) and the procedures (including fixation for electron microscopy) by which the resulting cultures can be exploited for analysis of hematopoietic cell interactions.

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V. References

  1. Leighton, J.; Mark, R.; Jasth, G. Patterns of threedimensional growth in vitro in collagen-coated cellulose sponge: Carcinoma and embryonic tissues. Cancer Res. 28: 286–296; 1968.

    PubMed  Google Scholar 

  2. Douglas, W. H. J.; McAtteer, J. A.; Cavanagh, T. Organotypic culture of dissociated fetal rat lung cells on a collagen sponge matrix. Procedure 44166. TCA Manual 4: 749–753; 1978.

    Google Scholar 

  3. Daniels, E.; Moore, K. L. A technic for studying self-disaggregation and self-reaggregation of embryonic tissues (abstr.). Anat. Rec. 163: 174; 1969.

    Google Scholar 

  4. Daniels, E. Growth and morphology of marrow stromal cells cultured in a three-dimensional support (abstr.). Anat. Rec. 187: 562; 1977.

    Google Scholar 

  5. Daniels, E. Ultrastructural observations on the three-dimensional reorganization and growth of newly-formed candidate stromal cells and residual hemopoietic cells in culture. Exp. Hematol. 8: 157–165; 1980.

    PubMed  Google Scholar 

  6. Daniels, E. Synthesis and distribution of3 H-fucose-containing glycoproteins by marrow stromal cells co-cultured with hemopoietic cells in gelfoam sponges: an electron microscope radioautographic study (abstr.). Exp. Hematol. 7: 20; 1979.

    Google Scholar 

  7. Grobstein, C. Tissue interactions in the morphogenesis of mouse embryonic rudiments in vitro. Aspects of synthesis and order in growth. Princeton, New Jersey: Princeton University Press; 1954: 233–256.

    Google Scholar 

  8. Metcalf, D.; Moore, M. A. S. Haemopoietic cells. Amsterdam-London: North Holland Publishing Company; 1971: 37–39.

    Google Scholar 

  9. Patt, H. M.; Maloney, M. A. Bone marrow regeneration after local injury: A review. Exp. Hematol. 3: 135–148; 1975.

    PubMed  Google Scholar 

  10. Chan, S. H.; Metcalf, D. Local production of colony stimulating factor within the bone marrow: Role of non-haemato-poietic cells. Blood 40: 646–653; 1972.

    PubMed  Google Scholar 

  11. Lord, B. I.; Mori, K. J.; Wright, E. G. A stimulator of stem cell proliferation in regenerating bone marrow. Biomedicine 27: 223–226; 1977.

    PubMed  Google Scholar 

  12. Daniels, E. The isolation of newly formed bone associated cells in vitro: Preliminary observations on origin and morphology. Exp. Hematol. 6: 48–57; 1978.

    PubMed  Google Scholar 

  13. Lord, B. I.; Hendry, J. H. The distribution of hematopoietic colony forming units in the mouse femur and its modification by X-rays. Brit. J. Radiol. 45: 110–115; 1972.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, McGill University, 3640 University Street, H3A 2B2, Montreal, Quebec, Canada

    E. Daniels

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  1. E. Daniels
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Daniels, E. Three-dimensional analysis of hemopoietic cellular interactions in vitro using gelfoam sponge matrices. Journal of Tissue Culture Methods 6, 65–69 (1980). https://doi.org/10.1007/BF01666019

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