Summary
An improved method for the preparation of chromosome spreads of slide cultured human diploid (MRC-5) cells is described. The technique provides large numbers of chromosome preparations suitable for counting and analysis, which is required for vaccine production from MRC-5 cells.
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References
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Knight, D.J., Musgrove, N.J. Method for preparing large numbers of MRC-5 cells on slides suitable for karyological analyses. Journal of Tissue Culture Methods 7, 81–83 (1982). https://doi.org/10.1007/BF01665915
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DOI: https://doi.org/10.1007/BF01665915