Summary
A protein assay method is described that involves simultaneous dye binding and precipitation of protein. It has a sensitivity comparable to the widely used Lowry and Bradford methods without the chemical interferences sometimes encountered with these methods in tissue culture conditions. The assay uses readily available reagents and is applicable to a variety of cell preparations.
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Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 193: 265–275; 1951.
Pesce, M. A.; Strande, C. S. A new micromethod for determination of protein in cerebrospinal fluid and urine. Clin. Chem. 19: 1265–1267; 1973.
Reuben, D. B.; Wachtel, T. J.; Brown, P. C.; Driscoll, J. L. Transient proteinuria in emergency medical admissions. N. Engl. J. Med. 306: 1031–1033; 1982.
Jauregui, H. O.; Hayner, N. T.; Laliberte, R.; Lipsky, M.; McMillan, P.; Galletti, P. M. Procurement of hepatocytes for a hybrid artificial liver. Trans. Am. Soc. Artif. Intern. Organs 25: 487–492; 1979.
Lozzio, C. B.; Lozzio, B. B. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321–334; 1975.
Bradford, M. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254; 1976.
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Hayner, N.T., Driscoll, J., Ferayorni, L. et al. Ponceau S: A sensitive method for protein determination in freshly isolated and cultured cells. Journal of Tissue Culture Methods 7, 77–80 (1982). https://doi.org/10.1007/BF01665914
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DOI: https://doi.org/10.1007/BF01665914