Summary
A differential plating method permitted preparation of cultures significantly enriched for Type II pneumocytes. These cells were maintained in a differentiated state for at least 12 d, identifiable morphologically (by presence of osmiophilic lamellar inclusion bodies) and bio-chemically (by demonstration of synthesis of phosphatidyl choline and production of disaturated lecithin).
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Shea, T.B., Keichline, L.D. A simple method for obtaining cultures enriched for Type II pneumonocytes from fetal rabbit. Journal of Tissue Culture Methods 7, 63–68 (1982). https://doi.org/10.1007/BF01665911
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DOI: https://doi.org/10.1007/BF01665911