Summary
Preparations of8H-monoacetylated derivatives ofNaja naja siamensis neurotoxinsiamensis 3 were found to be resistant to degradation and deacetylation duringin vitro muscle incubation. A low rate of free3H-acetate (<0.4%) was present in the preparations, but should not interfere with the binding of toxin to cholinergic receptors in mouse extensor digitorum longus musclesin vitro. The binding of toxin to cholinergic receptors in mouse extensor digitorum longus muscle was essentially irreversible. d-Tubocurarine antagonized the binding of toxin in both innervated and denervated muscles. Administration of actinomycin D one day after denervation prevented the appearance of new toxin binding sites in the muscles.
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Libelius, R., Eaker, D. & Karlsson, E. Further studies on the binding properties of cobra neurotoxin to cholinergic receptors in mouse skeletal muscle. J. Neural Transmission 37, 165–174 (1975). https://doi.org/10.1007/BF01663631
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DOI: https://doi.org/10.1007/BF01663631