Summary
Thein vitro activity of ofloxacin was tested against freshly isolated strains, from clinical material, ofPseudomonas aeruginosa, Staphylococcus aureus, and coagulase-negative staphylococci, including strains that were resistant to methicillin and/or novobiocin. 97% of thePseudomonas strains were inhibited by ofloxacin concentrations in the range of 0.78 to 6.25 mg/l, with 55% of the strains being inhibited by concentrations up to 1.56 mg/l. All of the staphylococci strains, including those resistant to novobiocin and/or methicillin, were sensitive to ofloxacin in the range 0.19 to 1.56 mg/l. The influence of ofloxacin on cellular and humoral host immunity was evaluated bothin vivo andin vitro. The influence of the compound on phagocytic activity of human polymorphonuclear leucocytes was evaluated by luminol-enhanced chemiluminescence. Ofloxacin did not influence cell-mediated immunity. It had no effect on chemotaxis. The use of sub-inhibitory concentrations of ofloxacin enhanced the phagocytic kill rate.
Zusammenfassung
DieIn vitro-Aktivität von Ofloxacin gegenüber frischen klinischen Isolaten vonPseudomonas aeruginosa, Staphylococcus aureus und koagulasenegativen Staphylokokken einschließlich Methicillin- und/oder Novobiocin-resistenter Stämme wurde geprüft. 97% der Pseudomonas-Stämme wurden durch Ofloxacin in Konzentrationen von 0,78 bis 6,25 mg/l gehemmt, 55% der Stämme bei Konzentrationen von bis zu 1,56 mg/l. Alle getesteten Staphylokokkenstämme einschließlich der Novobiocin- und/oder Methicillin-resistenten Stämme waren empfindlich gegen Ofloxacin in Konzentrationen von 0,19 bis 1,56 mg/l. Der Einfluß von Ofloxacin auf zelluläre und humorale Funktionen der körpereigenen Abwehr wurdein vivo undin vitro untersucht. Die Frage nach einer Beeinflussung der phagozytären Aktivität menschlicher polymorphkerniger Granulozyten wurden mittels Luminol-verstärkter Chemilumineszenz geprüft. Ofloxacin zeigte keinen Einfluß auf die zellvermittelte Immunität und auf die Chemotaxis. In subinhibitorischen Konzentrationen verstärkte Ofloxacin die Abtötungsrate durch phagozytierende Zellen.
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Pulverer, G., Peters, G. Investigations on ofloxacin: Antibacterial activity and influence on the immune system. Infection 14 (Suppl 4), S245–S247 (1986). https://doi.org/10.1007/BF01661283
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DOI: https://doi.org/10.1007/BF01661283