Summary
WhenA. ureafaciens are grown in a medium containing either creatinine or creatine as the sole source of carbon, an enzyme system capable of catabolizing creatine and creatinine is induced. This enzyme system has been isolated in a cell-free extract and is composed of two separate enzymes. The first, creatinine hydrolase, interconverts creatinine and creatine to form an equilibrium mixture of the two. The second enzyme, creatine amidinohydrolase, splits creatine into equimolar amounts of sarcosine and urea. The former enzyme is heat stable at 55°C for 30 min while the latter enzyme is completely destroyed at this temperature. The two enzymes have different solubilities in ammonium sulfate solutions.
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Predoctoral Fellow supported by NIH Training Grant GM00052.
Submitted in partial fulfillment of the M.S. degree.
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Kaplan, A., Naugler, D. Creatinine hydrolase and creatine amidinohydrolase: I. Presence in cell-free extracts of arthrobacter ureafaciens. Mol Cell Biochem 3, 9–15 (1974). https://doi.org/10.1007/BF01660072
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DOI: https://doi.org/10.1007/BF01660072