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The cytidine mechanism and methylation pathway in the formation of N-methylated ethanolamine phosphoglycerides in Neurospora crassa

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Summary

InNeurospora crassa, phospholipids containing ethanolamine, monomethylaminoethanol, dimethylaminoethanol and choline are formed by a pathway involving cytidine nucleotides. The rate of formation of these phospholipids does not appear to be dependent on the fatty acid composition of diacylglycerols acting as acceptors of phosphorylated aminoethanols. The presence of choline in the growth medium represses the activity of enzymes of the methylation pathway for the biosynthesis of 1,2-diacyl-sn-glycero-3-phosphorylcholine (diacyl-GPCh) and induces the activity of enzymes of the cytidine mechanism.

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Abbreviations

PCh:

choline phosphate

PDMAE:

dimethylaminoethanol phosphate

PMMAE:

monomethylaminoethanol phosphate

PEa:

ethanolamine phosphate

Diacyl-GPEa:

1,2-diacyl-sn-glycero-3-phosphoryl-ethanolamine

diacyl-GPCh:

1,2-diacyl-sn-glycero-3-phosphorylcholine

diacyl-GPMMAE:

1,2-sn-glycero-3-phosphorylmonomethylethanolamine

diacyl-GPDMAE:

1,2-diacyl-sn-glycero-3-phosphoryldimethylethanolamine

PEa-cytidyltransferase, CTP:

ethanolaminephosphate cytidyltransferase/EC 2.7.7.14/

PCh-cytidyltransferase, CTP:

cholinephosphate cytidyltransferase/EC 2.7.7.15/

PEa-transferase, CDP-ethanolamine:

1,2-diglyceride ethanolaminephosphotransferase/EC 2.7.8.1/

PCh-transferase, CDP-choline:

1,2-diglyceride cholinephosphotransferase/EC 2.7.8.1/

SAM:

S-adenosylmethionine

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka St., 02-532, Warszawa, Poland

    Z. Matysiak, A. Radomińska-Pyrek & T. Chojnacki

Authors
  1. Z. Matysiak
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  2. A. Radomińska-Pyrek
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  3. T. Chojnacki
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Additional information

This paper is dedicated with respect and affection to Prof. Dr JózefHeller, the discoverer of insect inorganic pyrophosphates that led to our studies on the biosynthesis of phospholipids.

An invited article.

CDPCh, CDPMMAE, CDPMMAE and CDPEa are the CMP derivatives of the respective aminoethanol phosphates.

PMMAE-cytidyltransferase and PDMAE-cytidyltransferase are enzymic activities of the same type as the two former but assayed with PMMAE and PDMAE respectively.

PMMAE-transferase and PDMAE-transferase are enzymic activities of the same type as the two former but assayed with CDPMMAE and CDPDMAE respectively.

PMMAE-transferase and PDMAE-transferase are enzymic activities of the same type as the two former but assayed with CDPMMAE and CDPDMAE respectively.

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Matysiak, Z., Radomińska-Pyrek, A. & Chojnacki, T. The cytidine mechanism and methylation pathway in the formation of N-methylated ethanolamine phosphoglycerides in Neurospora crassa. Mol Cell Biochem 3, 143–151 (1974). https://doi.org/10.1007/BF01659186

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  • DOI: https://doi.org/10.1007/BF01659186

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