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K+-independent effects of valinomycin in photosynthetic systems

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Abstract

With chromatophores ofRhodospirillum rubrum, valinomycin inhibited electron transport in the presence or absence of K+. NH4Cl had no effect on photophosphorylation but uncoupled with valinomycin present. ATPase activity was stimulated by NH4Cl plus valinomycin but not by either alone. K+ partially reversed the inhibition of phosphorylation and the stimulation of ATPase by valinomycin plus NH4Cl.

With chloroplasts, valinomycin inhibited coupled but not basal electron transport. The inhibition was only partially reversed by uncouplers. Valinomycin stimulated the light-activated Mg2+-dependent ATPase similar to several uncouplers such as quinacrine, methylamine, and S-13. In addition, valinomycin inhibited delayed light emission and stimulated the H+/e− ratio. These contrasting activities in chloroplasts are not easily explained.

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Authors and Affiliations

  1. Charles F. Kettering Research Laboratory, 45387, Yellow Springs, Ohio

    Donald L. Keister & Norma Jean Minton

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  1. Donald L. Keister
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  2. Norma Jean Minton
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Contribution number 389 of the Charles F. Kettering Research Laboratory.

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Keister, D.L., Minton, N.J. K+-independent effects of valinomycin in photosynthetic systems. J Bioenerg Biomembr 1, 367–377 (1970). https://doi.org/10.1007/BF01654574

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