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Human papillomavirus and c-myc/c-erbB2 in uterine and vulvar lesions

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Summary

The aim of this study was to investigate papillomavirus (HPV)-DNA in precancer and cancer of the cervix, vulva, and endometrium by in situ/dot blot/ Southern blot hybridization and polymerase chain reaction (PCR).Myc/erbB-2 expression was examined by Northern blot analysis. PCR was the most sensitive HPV detection method, demonstrating HPV-DNA in all preinvasive and invasive cervical lesions (n=21) and most (3 of 4) vulvar carcinomas in contrast to an overall rate of 60% with other techniques. Particular phenotypes (adenoid cystic/basal cell carcinoma of the vulva, cervical adenocarcinoma) were found to contain HPV. Endometrium harboured HPV not only in two cases of cervical cancer, but also in 3 of 8 primary endometrial carcinomas and 3 of 8 non-malignant conditions. activation was confined to three squamous cell carcinomas, most markedly in one HPV-6-positive verrucous variant.ErbB-2 over-expression was only seen in one HPV-18 infected advanced endometrial tumour. Our findings point to a range of HPV-infected lesions broader than previously supposed and possible contributions of HPV-independent molecular events to carcinogenesis in this field.

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Authors and Affiliations

  1. Department of Gynaecological Histopathology, Clinics of Obstetrics and Gynaecology, University Hospital, Hamburg, Federal Republic of Germany

    Karin Milde-Langosch, Gabriele Becker & Thomas Löning

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  1. Karin Milde-Langosch
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  2. Gabriele Becker
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  3. Thomas Löning
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Milde-Langosch, K., Becker, G. & Löning, T. Human papillomavirus and c-myc/c-erbB2 in uterine and vulvar lesions. Vichows Archiv A Pathol Anat 419, 479–485 (1991). https://doi.org/10.1007/BF01650676

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  • DOI: https://doi.org/10.1007/BF01650676

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