Summary
The phenotypic characterization of lymphocyte subsets in relation to different clinical stages of HIV infection has mainly focussed on CD4 and CD8 cells. Some reports focus on expansion of activated T lymphocytes in AIDS patients. Yet there is no detailed knowledge whether such changes occur also in earlier stages of HIV infection. In order to describe the kinetics and possible pathogenetic meaning of this subset when related to all distinct chronologic stages, we performed two-color flow cytometric lymphocyte differentiation in 173 HIV-infected patients and 30 healthy controls. All subjects were classified according to the Walter Reed (WR) system. Our results show that a significant increase of activated T lymphocytes (CD3+HLA/DR+) occurs early, in WR1 and WR2, thus preceding the clinically relevant CD4 depletion. This increase is paralleled by an expansion of CD 8+Leu7+cytotoxic cells. We conclude, thatearly changes of lymphocyte subsets are detectable in addition to inversion of the CD4/ CD8 ratio. The possible pathogenetic meaning including the question of possible autoimmune mechanisms is discussed.
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Abbreviations
- AIDS:
-
acquired immunodeficiency syndrome
- ARC:
-
AIDS-related complex
- CD:
-
cluster of differentiation
- FITC:
-
fluorescein isothiocyanate
- HIV:
-
human immunodeficiency virus
- MAB:
-
monoclonal antibodies
- NK:
-
natural killer
- PBMC:
-
peripheral blood mononuclear cells
- PE:
-
phycoerythrin
- SEM:
-
standard error of the mean
References
Bogner JR, Matuschke A, Heinrich B, Eberle E, Goebel F-D (1988) Serum neopterin levels as predictor of AIDS. Klin Wochenschr 66:1015–1018
Dixon WJ (ed) (1985) BMDP statistical software. University of California Press, Berkeley
Gay D, Maddon P, Sekaly R, Talle MA, Godfrey M, Long E, Goldstein G, Chess L, Axel R, Kappler J, Marrack P (1987) Functional interaction between human T-cell protein CD4 and the major histocompatibility complex HLA-DR antigen. Nature 328:626–629
Golding H, Shearer GM, Hillman K, Lucas P, Manischewitz J, Zajac RA, Clerici M, Gress RE, Boswell RN, Golding B (1989) Common epitope in human immunodeficiency virus (HIV)I-gp41 and HLA class II elicits immunosuppressive autoantibodies capable of contributing to immune dysfunction in HIV I-infected individuals. J Clin Investig 83:1430–1435
Gratama JW, Naipal AMIH, Oosterveer MAP, Stijnen T, Kluin-Nelemans HC, Ginsel LA, den Ottolander GJ, Hekker AC, D'Amaro J, van der Giessen M, Tanke GJ (1987) Effects of herpes virus carrier status on peripheral T lymphocyte subsets. Blood 2:516–523
Gupta S, Licorish K, Garthfield G (1983) Lymphocytic subsets in the acquired immunodeficiency syndrome. Ann Int Med 99:566–567
Hansel TT (1987) Leucocyte typing — OKCD? LancetII:1382–1383
Kornfeld H, Cruikshank WW, Pyle SW, Berman JS, Center DM (1988) Lymphocyte activation by HIV-1 envelope glycoprotein. Nature 335:445–448
Ljunggren K, Karlsson A, Fenyö E-M, Jondal M (1988) NK and ADCC function in HIV-infected patients from different clinical stages. Abstract 2093, IV. International Conference on AIDS, Stockholm 1988
Raffael A, Teufel E, Nerl C, Lander T (1986) IMK und Durchflu\cytometrie. Laborpraxis 10:656–659
Redfield RR, Wright DC, Tramont EC (1986) The Walter Reed staging classification for HTLV-III/LAV infection. N Engl J Med 314:131–132
Schwartz K, Visscher BR, Detels R, Taylor J, Nishanian P, Fahey JL (1985) Immunological changes in lymphadenopathy virus positive and negative symptomless male homosexuals: two years of observation. Lancet II:831–832
Volk HD, Koyka I, Schmidt R, Baumgarten R, Breustedt W, Sönnichsen N, von Baehr R (1988) Changes in the subsets of lymphocytes and monocytes in the peripheral blood of individuals infected with HIV-1. AIDS-Forschung (AIFO) 3:350–354
Ziegler-Heitbrock HWL, Stachel D, Schlunk T, Gürtler L, Schramm W, Fröschl M, Bogner JR, Riethmüller G (1988) Class II (DR) antigen expression on CD8+ lymphocyte subsets in acquired immune deficiency syndrome (AIDS). J Clin Immunol 8:473–478
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Bogner, J.R., Matuschke, A., Heinrich, B. et al. Expansion of activated T lymphocytes (CD3+HLA/DR+) detectable in early stages of HIV-1 infection. Klin Wochenschr 68, 393–396 (1990). https://doi.org/10.1007/BF01648577
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DOI: https://doi.org/10.1007/BF01648577