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Comparative studies of avian DNA by restriction fragment length polymorphism analysis: Convenient procedures based on blood samples from live birds

Untersuchungen von Vogel-DNS zur Feststellung von Polymorphien in der Längenverteilung von Restriktionsfragmenten: Leicht anwendbare Methoden

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Summary

One of the new DNA techniques, restriction fragment length polymorphism (RFLP) analysis is outlined. Simple and low cost sampling and preservation procedures of DNA from live birds are presented, that make it feasible to collect large sample sizes. DNA extraction protocols are listed and it is reported that mitochondrial DNA can be analysed without having to kill the birds. The great potential in building up special collections of avian DNA is emphasized.

Zusammenfassung

Auf eine der neuen DNS-Methoden, „Restriktionsfragment-Längenpolymorphie-Analyse” (RFLP), wird näher eingegangen. Einfache und billige Konservierungsmethoden für DNS-Proben aus lebenden Vögeln werden beschrieben. Hierdurch wird es möglich, große Stichproben zu sammeln. Verfahren zur DNS-Extraktion werden angegeben. Insbesondere ist es möglich, Mitochondrien-DNS zu analysieren, ohne die Vögel töten zu müssen. Die zukünftigen Möglichkeiten, die sich aus der Anlage größerer Sammlungen von Vogel-DNS ergeben, werden herausgestellt.

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Authors and Affiliations

  1. Institute of Genetics, Univ. Øster Farimagsgade 2A, DK-1353, Copenhagen K, Denmark

    Peter Arctander

  2. Zool. Mus. Univ. Universitetsparken 15, DK-2100, Copenhagen Ø, Denmark

    Peter Arctander

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  1. Peter Arctander
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Arctander, P. Comparative studies of avian DNA by restriction fragment length polymorphism analysis: Convenient procedures based on blood samples from live birds. J Ornithol 129, 205–216 (1988). https://doi.org/10.1007/BF01647289

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