Skip to main content
SpringerLink
Log in

Colonization of Nigerian neonates with group B streptococci and its rapid detection

Besiedelung nigerianischer Neugeborener mit Streptokokken der Gruppe B und ihr Nachweis mit Schnelltests

  • Originalia
  • Published:
Infection Aims and scope Submit manuscript

Summary

The rate of colonization by group B streptococci among Nigerian neonates in Abeokuta was found to be 19% (38 of 200 newborns). The recently described serotype IV was the commonest (35.1%) among the strains isolated. Three methods for the rapid detection of heavy colonization by group B streptococci in neonates were compared: Gram stain of the pharyngeal aspirate collected immediately post-partum; direct application of latex agglutination to the first post-partum pharyngeal aspirate; and application of latex agglutination to selective Todd Hewitt broth, inoculated with pharyngeal aspirate and body swabs, after five hours of incubation. It seems that both latex agglutination methods can rapidly detect a considerable proportion of neonates heavily contaminated with group B streptococci intra-partum; therefore both methods deserve further evaluation.

Zusammenfassung

Die Besiedelungsrate nigerianischer Neugeborener mit Gruppe-B-Streptokokken betrug in Abeokuta 19% (38 von 200 Neugeborenen). Unter den Isolaten war der erst seit kurzem bekannte Serotyp IV der häufigste (35,1%). Drei Methoden zur Früherkennung der intensiven Besiedelung Neugeborener mit GBS wurden verglichen: Gramfärbung des gleich nach der Geburt abgesaugten Rachensekretes; direkte Anwendung eines Latex-Testes an diesem Rachensekret; Anwendung des Latex-Testes an selektiver Todd-Hewitt-Bouillon, die mit Rachensekret bzw. mit Körperabstrichen beimpft und fünf Stunden bebrütet worden war. Beide Latex-Methoden scheinen in der Lage zu sein, frühzeitig einen erheblichen Anteil der Neugeborenen zu erkennen, die während der Geburt intensiv mit Gruppe-B-Streptokokken kontaminiert wurden. Beide Methoden sollten deshalb ausführlicher evaluiert werden.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature

  1. Baker, C. J. Summary of the workshop on perinatal infections due to group B streptococcus. J. Infect. Dis. 136 (1977) 137–152.

    Article  Google Scholar 

  2. Dawodu, A. H., Damole, I. O., Onile, B. A. Epidemiology of group B streptococcal carriage among pregnant women and their neonates: an African experience. Trop. Geogr. Med. 35 (1983) 145–150.

    CAS  PubMed  Google Scholar 

  3. Onile, B. A. Group B streptococcal carriage in Nigeria. Trans. Roy. Soc. Trop. Med. Hyg. 74 (1980) 367–370.

    Article  CAS  PubMed  Google Scholar 

  4. Benchetrit, L. C., Fracalanzza, S. E. L., Peregrino, H., Camelo, A. A., Sanches, L. A. L. R. Carriage ofStreptococcus agalactiae in women and neonates and distribution of serological types: a study in Brazil. J. Clin. Microbiol. 15 (1982) 787–790.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Mayon-White, R. T. The incidence of GBS disease in neonates in different countries. In:Christensen, K. K., Christensen, P., Ferrieri, P. (eds.): Neonatal group B streptococcal infections. Antibiot. Chemother. 35 (1985) 17–27.

    Article  CAS  PubMed  Google Scholar 

  6. Hallett, A. F., Govender, P., Pillay, S. S., Cooper, R. Group B streptococci in blacks. S. Afr. Med. J. 55 (1979) 157–159.

    CAS  PubMed  Google Scholar 

  7. Thangavelu, C. P., Koshi, G. Group B streptococcal types from human infections in Southern India. In:Parker, M. T. (ed.): Pathogenic streptococci. Reedbooks, Chertsey, U.K. 1979, pp. 174–175.

    Google Scholar 

  8. Minhaj, A. A., Jamal, F., Mohamed, R. Neonatal group B beta haemolytic streptococcal infection in Kuala Lumpur. Med. J. Malaysia 35 (1980) 117–121.

    CAS  PubMed  Google Scholar 

  9. Kiong, C. K., Leng, T., Lam, C. A prospective study of group B streptococcal colonization in parturient mothers and their infants. Ann. Acad. Med. (Singapore) 10 (1981) 79–83.

    Google Scholar 

  10. Bromberger, P. I., Chandler, B., Gezon, H., Haddow, J. E. Rapid detection of neonatal group B streptococcal infections by latex agglutination. J. Pediatr. 96 (1980) 104–106.

    Article  CAS  PubMed  Google Scholar 

  11. Webb, B. J., Baker, C. J. Commercial latex agglutination test for rapid diagnosis of group B streptococcal infection in infants. J. Clin. Microbiol. 12 (1980) 442–444.

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Ingram, D. L., Suggs, D. M., Pearson, A. W. Detection of group B streptococcal antigen in early-onset and late-onset group B streptococcal disease with the Wellcogon Strep B latex agglutination test. J. Clin. Microbiol. 16 (1982) 656–658.

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Holmes, R. L., Harada, W. A. Rapid method for identification of group B streptococci in neonatal blood cultures. J. Clin. Microbiol. 13 (1981) 279–282.

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Lim, D. V., Kanarek, K. S., Peterson, M. E. Magnitude of colonization and sepsis by group B streptococci in newborn infants. Curr. Microbiol. 7 (1982) 99–101.

    Article  Google Scholar 

  15. Slack, M. P. E., Mayon-White, R. T. Group B streptococci in pharyngeal aspirates at birth and the early detection of neonatal sepsis. Arch. Dis. Childhood 53 (1978) 540–544.

    Article  CAS  Google Scholar 

  16. Ingram, D. L., Pendergrass, E. L., Bromberger, P. I., Thullen, J. D., Yoder, C. D., Collier, A. M. Group B streptococcal disease. Am. J. Dis. Child. 134 (1980) 754–758.

    Article  CAS  PubMed  Google Scholar 

  17. Jones, D. E., Friedl, E. M., Kanarek, K. S., Williams, J. K., Lim, D. V. Rapid identification of pregnant women heavily colonized with group B streptococci. J. Clin. Microbiol. 18 (1983) 558–560.

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Jones, D. E., Kanarek, K. S., Lim, D. V. Group B streptococcal colonization patterns in mothers and their infants. J. Clin. Microbiol. 20 (1984) 438–440.

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Ferrieri, P.: GBS infections in the newborn infant. In:Christensen, K. K., Christensen, P., Ferrieri, P. (eds.): Neonatal group B streptococcal infections. Antibiot. Chemother. 35 (1985) 211–224.

  20. Gray, B. M., Pass, M. A., Dillon, Jr. , H. C. Laboratory and field evaluation of selective media for isolation of group B streptococci. J. Clin. Microbiol. 9 (1979) 466–470.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Baker, C. J., Barrett, F. F. Transmission of group B streptococci among parturient women and their neonates. J. Pediatr. 83 (1973) 919–925.

    Article  CAS  PubMed  Google Scholar 

  22. Maillot, K. v., Rummel, W. Die Gefährdung des Neugeborenen durch die Scheidenflora der Mutter. Dtsch. Ärztebl. 41 (1975) 2844–2846.

    Google Scholar 

  23. Naor, N., Blumenfeld, Z., Merzbach, D., Timor-Trisch, I., Zeltzer, M. Prevalence of group B streptococci in parturient mothers and their infants. Eur. J. Pediatr. 138 (1982) 166–167.

    Article  CAS  PubMed  Google Scholar 

  24. Onile, B. A., Osinusi, B. O., Montefiore, D., Sogbetun, A. O. Neonatal septicaemia resulting from group B streptococci: a case report. Int. J. Gynaecol. Obstet. 17 (1980) 393–395.

    CAS  PubMed  Google Scholar 

  25. Baker, C. J., Kasper, D. L. Correlation of maternal antibody deficiency with susceptibility to neonatal group B streptococcal infection. N. Engl. J. Med. 294 (1976) 753–756.

    Article  CAS  PubMed  Google Scholar 

  26. Gotoff, S. P., Boyer, K. M.: Cellular and humoral aspects of host defense mechanisms against GBS. In:Christensen, K. K., Christensen, P., Ferrieri, P. (eds.): Neonatal group B streptococcal infections. Antibiot. Chemother. 35 (1985) 142–156.

  27. Jelínková, J., Motlová, J. Worldwide distribution of two new serotypes of group B streptococci: type IV and provisional type V. J. Clin. Microbiol. 21 (1985) 361–362.

    PubMed  PubMed Central  Google Scholar 

  28. Doskeland, S. O., Berdal, B. P. Bacterial antigen detection in body fluids: methods for rapid antigen concentration and reduction of nonspecific reactions. J. Clin. Microbiol. 11 (1980) 380–384.

    CAS  PubMed  PubMed Central  Google Scholar 

  29. Jones, D. E., Kanarek, K. S., Angel, J. L., Lim, D. V. Elimination of multiple reactions of the Phadebact Streptococcus coagglutination test. J. Clin. Microbiol. 18 (1983) 526–28.

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Institute of Hygiene, P. O. Box 1729, D-7400, Tübingen, Germany

    J. E. Hoppe

  2. Institute of Tropical Medicine, Eberhard Karl University, D-7400, Tübingen, Germany

    J. Grieshaber &  W. Höfler

Authors
  1. J. E. Hoppe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Grieshaber
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Höfler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hoppe, J.E., Grieshaber, J. & Höfler, W. Colonization of Nigerian neonates with group B streptococci and its rapid detection. Infection 14, 74–78 (1986). https://doi.org/10.1007/BF01644447

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01644447

Keywords

Search

Navigation