Summary
Paternity testing was carried out in 271 cases of disputed paternity using the 5 VNTR systems D2S44 (YNH24), D5S43 (MS8), D7S21 (MS31), D7S22 (g3), and D12S11 (MS43a), and 10–15 conventional marker systems including the HLA-A,B system. By means of the matching criteria for the VNTR systems established elsewhere (Morling & Hansen 1992), all 70 unrelated men who had been excluded by conventional typing were also excluded with 2 or more VNTR systems. Based on the observed exclusion frequencies for the 5 VNTR systems, a theoretical exclusion rate exceeding 0.999 could be obtained. A total of 350 father/child pairs were studied and in 3 paternity cases and one immigrant family, the alleged fathers were excluded solely by one of the 5 VNTR systems possibly reflecting mutations. No mother/child exclusions were observed among 350 mother/child pairs. Linkage analysis between the syntenic systems D7S21 (MS31) and D7S22 (g3) was performed in 29 informative families with 81 children and revealed a recombination distance of about 31 cM. The positive evidence for paternity provided by the 5 VNTR systems in cases with non-exclusions is discussed.
Zusammenfassung
Vaterschaftstests wurden in 271 Fällen strittiger Vaterschaft mit Hilfe der 5 VNTR-Systeme: D2S44 (YNH24), D5S43 (MS8), D7S21 (MS31), D7S22 (g3), D12S11 (MS43a) und 12–15 konventionellen Blutgruppensystemen einschließlich des HLA-Systems (HLA A und B) durchgeführt. Mit Hilfe der Matching-Kriterien für VNTRSysteme, wie sie andernorts (Morling und Hansen 1992) etabliert wurden, wurden alle 70 unverwandten Männer, die durch konventionelle Typisierung ausgeschlossen wurden, auch mit Hilfe von 2 oder mehreren VNTR-Systemen ausgeschlossen. Basierend auf den beobachteten Ausschlußfrequenzen für die 5 VNTR-Systeme könnte eine theoretische Ausschlußwahrscheinlichkeit von mehr als 0,999 erhalten werden. Insgesamt wurden 350 Vater/Kind-Paare untersucht und in 3 Vaterschaftsfällen und einer Emigrantenfamilie wurden isolierte Ausschlüsse der Putativväter ausschließlich in einem der 5 VNTR-Systeme gefunden, was möglicherweise an Mutationen denken läßt. Unter 350 Mutter/Kind-Paaren wurden keine Mutter/Kind-Ausschlüsse gefunden. Linkage-Analysen zwischen den Syntenic-Systemen D7S21 (MS31) und D7S22 (g3) wurden durchgeführt an 29 Familien mit 81 Kindern. Die Analyse ergab eine Rekombinationsdistanz von ungefähr 31 cM. Der positive Vaterschaftsbeweis, wie er durch die 5 VNTR-Systeme in Fällen mit Nicht-Ausschlüssen etabliert wird, wird diskutiert.
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Hansen, H.E., Morling, N. Paternity testing with VNTR DNA systems. Int J Leg Med 105, 197–202 (1993). https://doi.org/10.1007/BF01642793
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DOI: https://doi.org/10.1007/BF01642793