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Ordered distribution of α-putrescinylthymine in the DNA of bacteriophage φW-14

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Abstract

In the DNA of bacteriophage ϕW-14, half the thymine is replaced by a α-putrescinylthymine (putThy). Analysis of monopyrimidine tracts shows that putThy and thymine are distributed nonrandomly in φW-14 DNA: The sequence purine-putThy-purine occurs more than twice as frequently as the sequence purine-thymine-purine, which means that the post-replicative modification of φW-14 DNA is sequence-specific.

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Literature Cited

  1. Burton, K., Petersen, G. B. 1960. The frequencies of certain sequences of nucleotides in deoxyribonucleic acid. Biochemical Journal75:17–27.

    PubMed  Google Scholar 

  2. Dosmar, M., Witmer, H. 1979. An atypical deoxynucleoside triphosphate inBacillus subtilis infected with bacteriophage SP10c. Current Microbiology2:361–364.

    Google Scholar 

  3. Flink, I., Pettijohn, D. E. 1975. Polyamines stabilize DNA folds. Nature253:62–63.

    PubMed  Google Scholar 

  4. Kelln, R. A., Warren, R. A. J. 1973. Studies on the biosynthesis of α-putrescinylthymine in bacteriophage φW-14-infectedPseudomonas acidovorans. Journal of Virology12:1427–1433.

    PubMed  Google Scholar 

  5. Kropinski, A. M. B., Bose, R. J., Warren, R. A. J. 1973. 5-(4-Aminobutyl-aminomethyl)uracil, an unusual pyrimidine from the DNA of bacteriophage φW-14. Biochemistry12:151–157.

    PubMed  Google Scholar 

  6. Lunt, M. R., Siebke, J.-C., Burton, K. 1964. Glucosylated nucleotide sequences from T2-bacteriophage deoxyribonucleic acid. Biochemical Journal92:27–36.

    PubMed  Google Scholar 

  7. Maltman, K. L., Neuhard, J., Lewis, H. A., Warren, R. A. J. 1980. Synthesis of thymine and α-putrescinylthymine in bacteriophage φW-14-infectedPseudomonas acidovorans. Journal of Virology34:354–359.

    PubMed  Google Scholar 

  8. Maltman, K. L., Neuhard, J., Warren, R. A. J. 1981. 5-(Hydroxymethyl-O-pyrophosphoryl)uracil, an intermediate in the biosynthesis of α-putrescinlythymine in the DNA of bacteriophage φW-14. Biochemistry20:3586–3591.

    PubMed  Google Scholar 

  9. Rae, P. M. M. 1973. 5-Hydroxymethyluracil in the DNA of a dinoflagellate. Proceedings of the National Academy of Sciences, U.S.A.70:1141–1145.

    Google Scholar 

  10. Randerath, K. 1969. An improved procedure for solid scintillation fluorography of tritium compounds. Analytical Chemistry41:991–992.

    Google Scholar 

  11. Revel, H. R., Luria, S. E. 1970. DNA glucosylation in T-even phage: Genetic determination and role in phage-host interaction. Annual Review of Genetics4:177–192.

    PubMed  Google Scholar 

  12. Shapiro, H. S., Chargaff, E. 1963. Studies on the nucleotide arrangement in deoxyribonucleic acids VII. Direct estimation of pyrimidine nucleotide runs. Biochimica et Biophysica Acta76:1–8.

    PubMed  Google Scholar 

  13. Steele, R. E., Rae, P. M. M. 1980. Ordered distribution of modified bases in the DNA of a dinoflagellate. Nucleic Acids Research8:4709–4725.

    PubMed  Google Scholar 

  14. Warren, R. A. J. 1980. Modified bases in bacteriophage DNAs. Annual Review of Microbiology34:137–158.

    PubMed  Google Scholar 

  15. Witmer, H., Dosmar, M. 1978. Synthesis of 5-hydroxymethyldeoxyuridine triphosphate in extracts of SP10c phage-infectedBacillus subtilis W23. Current Microbiology1:289–292.

    Google Scholar 

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  1. Department of Microbiology, University of British Columbia, V6T 1W5, Vancouver, British Columbia, Canada

    Richard A. J. Warren

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  1. Richard A. J. Warren
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Warren, R.A.J. Ordered distribution of α-putrescinylthymine in the DNA of bacteriophage φW-14. Current Microbiology 6, 185–188 (1981). https://doi.org/10.1007/BF01642396

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