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Genetic relatedness of clinical and environmental isolates of lactose-positiveVibrio vulnificus

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Abstract

Environmental isolates of lactose-positiveVibrio vulnificus from different geographic areas were compared with clinical strains ofV. vulnificus on the basis of their phenotypic traits, virulence, DNA base composition, and DNA-DNA reasociation. EnvironmentalV. vulnificus strains were phenotypically indistinguishable from clinical isolates. These strains had a DNA base composition of 47–48 mol% guanine + cytosine and ≥85% reassociation at stringent temperature with DNA from clinicalV. vulnificus strains. These result indicateV. vulnificus strains from widely separated regions of the marine environment are indistinguishable from strains that have been agents of septicemia associated with shellfish consumption and of wound infections associated with seawater exposure.

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Literature Cited

  1. Baumann, P., Baumann, L., Bang, S. S., Woolkalis, M. J. 1980. Reevaluation of the taxonomy ofVibrio, Beneckea, andPhotobacterium: Abolition of the genusBeneckea. Current Micribiology4:127–132.

    Google Scholar 

  2. Blake, P. A., Merson, M. H., Weaver, R. E., Hollis, D. G., Heublein, P. C. 1979. Disease caused by a marineVibrio: Clinical characteristics and epidemiology. New England Journal of Medicine300:1–5.

    PubMed  Google Scholar 

  3. Brenner, D. J., Fanning, G. R., Johnson, K. E., Citarella, R. V., Falkow, S. 1969. Polynucleotide sequence relationships among members ofEnterobacteriaceae. Journal of Bacteriology98:637–650.

    PubMed  Google Scholar 

  4. Clark, W. A., Steigerwalt, A. G. 1977. Deoxyribonucleic acid reassociation experiments with a halophilic, lactosefermenting vibrio isolated from blood cultures. International Journal of Systematic Bacteriology27:194–199.

    Google Scholar 

  5. Farmer, J. J., III 1979.Vibrio (“Beneckea”) vulnificus, the bacterium associated with sepsis, septicemia, and the sea. Lancet2(2):902.

    Google Scholar 

  6. Hollis, D. G., Weaver, R. E., Baker, C. N., Thornsberry, C. 1976. HalophilicVibrio species isolated from blood cultures. Journal of Clinical Microbiology3:425–431.

    Google Scholar 

  7. Johnson, J. L., Ordal, E. J. 1968. Deoxyribonucleic acid homology in bacterial taxonomy: Effect of incubation temperature on reaction specificity. Journal of Bactaeriology95:893–900.

    Google Scholar 

  8. Kelly, M. T., Avery, D. M. 1980. Lactose-positiveVibrio in seawater: A cause of pneumonia and septicemia in a drowning victim. Journal of Clinical Microbiology11:278–280.

    PubMed  Google Scholar 

  9. Mandel, M., Igambi, L., Bergendahl, J., Dodson, M. L., Jr., Scheltgen, E. 1970. Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. Journal of Bacteriology101:333–338.

    PubMed  Google Scholar 

  10. Oliver, J. D., Cleland, D. R., Warner, R. A. 1981. Lactosefermenting vibrios in the marine environment. Abstracts of the Annual Meeting of the American Society for Microbiology1981:175.

    Google Scholar 

  11. Reichelt, J. L., Baumann, P., Baumann, L. 1976. Study of genetic relationships among marine species of the generaBeneckea andPhotobacterium by means ofin vitro DNA/DNA hybridization. Archives of Microbiology110:101–120.

    PubMed  Google Scholar 

  12. Shewan, J. M., Véron, M. 1971. Genus I.Vibrio Pacini 1854, 411, pp. 340–345. In: Buchanan, R. E., Gibbons, N. E. (eds.), Bergey's manual of determinative bacteriology, 8th ed. Baltimore: Williams and Wilkins.

    Google Scholar 

  13. Sriranganathan, N., Seidler, R. J., Sandine, W. E., Elliker, P. R. 1973. Cytological and deoxyribonucleic acid-deoxyribonucleic acid hybridization studies onLactobacillus isolates from San Francisco sourdough. Applied Microbiology25:461–470.

    PubMed  Google Scholar 

  14. Tison, D. L., Nishibuchi, M., Seidler, R. J. 1981. Isolation and characterization of potentially pathogenic vibrios from Oregon estuaries. Abstract of the Annual Meeting of the American Society for Microbiology1981:216.

    Google Scholar 

  15. Woodward, B. W., Carter, M., Seidler, R. J. 1979. Most nonclinicalKlebsiella strains are notK. pneumoniae sensu stricto. Current Microbiology2:181–185.

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Oregon State University, 97331, Corvallis, Oregon, USA

    David L. Tison & Ramon J. Seidler

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  1. David L. Tison
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  2. Ramon J. Seidler
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Tison, D.L., Seidler, R.J. Genetic relatedness of clinical and environmental isolates of lactose-positiveVibrio vulnificus . Current Microbiology 6, 181–184 (1981). https://doi.org/10.1007/BF01642395

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