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Isolation and characterization of the nuclei fromSaccharomyces cerevisiae

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Abstract

We isolated highly intact nuclei from cells of a haploid strain ofSaccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

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Authors and Affiliations

  1. Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Sendamachi 3, Naka-ku, 730, Hiroshima, Japan

    Kazuo Kamimura, Eiko Tsuchiya, Tokichi Miyakawa & Sakuzo Fuki

  2. Institute of Applied Microbiology, The University of Tokyo, Yayoi 1, Bunkyo-ku, 113, Tokyo, Japan

    Aiko Hirata

Authors
  1. Kazuo Kamimura
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  2. Eiko Tsuchiya
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  3. Tokichi Miyakawa
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  4. Sakuzo Fuki
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  5. Aiko Hirata
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Kamimura, K., Tsuchiya, E., Miyakawa, T. et al. Isolation and characterization of the nuclei fromSaccharomyces cerevisiae . Current Microbiology 6, 175–180 (1981). https://doi.org/10.1007/BF01642394

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  • DOI: https://doi.org/10.1007/BF01642394

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