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Enzymatische Wertbestimmung von Aminoglykosid-Antibiotika

Enzymatic assays of aminoglycoside antibiotics

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Zusammenfassung

Mit bakteriellen Enzymen, die Aminoglykosid-Antibiotika adenylylieren oder acetylieren, lassen sich Konzentrationen dieser Chemotherapeutika im Serum und anderen Körperflüssigkeiten von Patienten bestimmen. Die enzymatischen Teste stellen genaue, stabile Mikromethoden dar, deren Ergebnisse durch andere Antibiotika nicht beeinflußt werden und die rasch und preiswert durchzuführen sind.

Summary

Adenylylating or acetylating enzymes, which are produced by grampositive and gramnegative bacteria resistant to aminoglycoside antibiotics, are useful tools in determining concentrations of aminoglycosides in patients' body fluids. Due to their accuracy, stability, versatility, fastness and inexpensiveness, enzymatic assays are the tests of choice for the routine determination of aminoglycoside concentrations.

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Literatur

  1. Benveniste, R., Davies, J. Mechanisms of antibiotic resistance in bacteria. Ann. Rev. Biochem. 42 (1973) 472–506.

    Article  Google Scholar 

  2. Broughall, J. M., Reeves, S. Properties of the gentamicin acetyltransferase enzyme and application to the assay of aminoglycoside antibiotics. Antimicrob. Ag. Chemother. 8 (1975) 222–223.

    Article  CAS  Google Scholar 

  3. Daignueault, R., Gagne, M., Brazeau, M. A comparison of two methods of gentamicin assay: An enzymatic procedure and an agar diffusion technique. J. Infect. Dis. 130 (1974) 642–645.

    Article  Google Scholar 

  4. Davies, J., Brzezinska, M., Benveniste, R. R-factors: Biochemical mechanisms of resistance to aminoglycoside antibiotics. Ann. N.Y. Acad. Sci. 182 (1971) 494–499.

    Article  Google Scholar 

  5. Furger, F., Russi, A., Kayser, F. H. Schnellmethoden zur quantitativen Bestimmung von Aminoglykosid-Antibiotika. Schweiz. med. Wschr. 103 (1973) 779–782.

    CAS  PubMed  Google Scholar 

  6. Haas, M. J., Davies, J. Enzymatic acetylation as a means of determining serum aminoglycoside concentrations. Antimicrob. Ag. Chemother. 4 (1973) 497–499.

    Article  CAS  Google Scholar 

  7. Holmes, R. K., Sanford, J. P. Enzymatic assay for gentamicin and related aminoglycoside antibiotics. J. infect. Dis. 129 (1974) 519–527.

    Article  CAS  PubMed  Google Scholar 

  8. Mosegaard, A., Welling, P. G., Madsen, P. O. Gentamicin and gentamicin C1 in the treatment of complicated urinary tract infections: Comparative study of efficacy, tolerance and pharmacokinetics. Antimicrob. Ag. Chemother. 7 (1975) 328–332.

    Article  CAS  Google Scholar 

  9. Santanam, P., Kayser, F. H. Tobramycin-adenylyltransferase: A new aminoglycoside inactivating enzyme from Staphylococcus epidermidis. J. infect. Dis. 134 (1976) S33-S39.

    Article  PubMed  Google Scholar 

  10. Santanam, P., Kayser, F. H. Enzymatic adenylylation by aminoglycoside 4′-adenylyltransferase and 2″-adenylyltransferase as a means of determining serum level of aminoglycoside antibiotics. Antimicrob. Ag. Chemother. 10 (1976) 664–667.

    Article  CAS  Google Scholar 

  11. Schering Corporation: Professional Brochure. 1974.

  12. Shaw, W. V., Carter, J., Sachs, J. Enzymatic assay of gentamicin and kanamycin in body fluids. Clin. Res. 20 (1972) 83.

    Google Scholar 

  13. Smith, A. L., Smith, D. H. Gentamicin: Adenine mononucleotide transferase: Partial purification, characterization, and use in the clinical quantitation. J. infect. Dis. 129 (1974) 391–401.

    Article  CAS  PubMed  Google Scholar 

  14. Stevens, P., Young, L. S., Hewitt, W. L. Improved acetylating radioenzymatic assay of amikacin, tobramycin and sisomicin in serum. Antimicrob. Ag. Chemother. 7 (1975) 374–376.

    Article  CAS  Google Scholar 

  15. Williams, J. W., Langer, J. S., Northrop, D. B. A spectrophotometric assay for gentamicin. J. Antibiot. (Tokyo) 28 (1975) 982–987.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut für Medizinische Mikrobiologie der Universität, Postfach, CH-8028, Zürich, Schweiz

    F. H. Kayser

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  1. F. H. Kayser
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Kayser, F.H. Enzymatische Wertbestimmung von Aminoglykosid-Antibiotika. Infection 5, 123–125 (1977). https://doi.org/10.1007/BF01642097

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  • DOI: https://doi.org/10.1007/BF01642097

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