Skip to main content
SpringerLink
Log in

Antibodies against an acid extract from a single Campylobacter strain in hospitalized campylobacter patients

Antikörper gegen den Säureextrakt von einem einzelnen Campylobacter-Stamm bei hospitalisierten Patienten mit Campylobacter-Infektionen

  • Originalia
  • Published:
Infection Aims and scope Submit manuscript

Summary

An acid glycine extract from a singleCampylobacter jejuni strain was used as the antigen in the enzyme immunoassay (EIA). Elevated values indicating the presence of specific IgM and/or IgG antibodies were found in 73% of 52 patients hospitalized for campylobacter infection. A further 6% of the patients who had lower EIA values in paired serum samples showed at least a 20% change in EIA values. The same sera had been studied earlier for agglutinating antibodies using four reference campylobacter strains. 74% of the patients had shown elevated titres and a further 17% four-fold changes of lower titres in paired sera.

Zusammenfassung

Im Enzym-Immunassay (EIA) wurde ein Säure-Glycin-Extrakt von einem einzelnen Stamm vonCampylobacter jejuni als Antigen verwendet. Bei 73% von 52 wegen Campylobacter-Infektionen stationär behandelten Patienten lagen die Antikörperwerte in einem Bereich, der auf Vorhandensein spezifischer IgM- und/oder IgG-Antikörper schließen läßt. Bei weiteren 6% der Patienten wurden niedrigere EIA-Werte gemessen, doch zeigten sich in gepaarten Serumproben Unterschiede in den EIA-Werten von mindestens 20%. Dieselben Seren waren in Voruntersuchungen auf agglutinierende Antikörper mit vier Campylobacter-Referenzstämmen getestet worden. Eindeutige Titererhöhungen fanden sich bei 74% der Patienten; 17% der Patienten wiesen niedrigere Titer auf, doch waren in gepaarten Seren Unterschiede von mindestens vier Verdünnungsstufen festzustellen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature

  1. Watson, K. C., Kerr, E. J. C., McFadzean, S. M. Serology of human campylobacter infections. J. Infection 1 (1979) 151–158.

    Google Scholar 

  2. Kosunen, T. U., Danielsson, D., Kjellander, J. Serology ofCampylobacter fetuss ss.jejuni (“Related” Campylobacters). Acta Pathol. Microbiol. Scand. (B) 88 (1980) 207–218.

    Google Scholar 

  3. Penner, J. L., Hennessy, J. N. Passive hemagglutination technique for serotypingCampylobacter fetus subsp.jejuni on the basis of soluble heat-stable antigens. J. Clin. Microbiol. 12 (1980) 732–737.

    Google Scholar 

  4. Lauwers, S., Vlaes, L., Butzler, J. P. Campylobacter serotyping and epidemiology. Lancet I (1981) 158–159.

    Google Scholar 

  5. Skirrow, M. B. Campylobacter enteritis: a “new” disease. Br. Med. J. 2 (1977) 9–11.

    Google Scholar 

  6. Blaser, M. J., Berkowitz, I. D., LaForce, F. M., Cravens, J., Reller, L. B., Wang, W. L. Campylobacter enteritis: clinical and epidemiological features. Ann. Intern. Med. 91 (1979) 179–185.

    Google Scholar 

  7. Karmali, M. A., Fleming, P. C. Campylobacter enteritis in children. J. Pediatr. 94 (1979) 527–533.

    Google Scholar 

  8. Jones, D. M., Eldridge, J., Dale, B. Serological response toCampylobacter jejuni/coli infection. J. Clin. Pathol. 33 (1980) 767–769.

    Google Scholar 

  9. Kosunen, T. U., Pitkänen, T., Pettersson, T., Pönkä, A. Clinical and serological studies in patients withCampylobacter fetus ssp.jejuni infection: II. Serological findings. Infection 9 (1981) 279–282.

    Google Scholar 

  10. McCoy, E. C., Doyle, D., Burda, K., Corbeil, L. B., Winter, A. J. Superficial antigens ofCampylobacter (Vibrio) fetus: Characterization of an antiphagocytic component. Infect. Immun. 11 (1975) 517–525.

    Google Scholar 

  11. Voller, A., Bridwell, D., Bartlett, A. Microplate enzyme immunoassays for the immunodiagnosis of virus infections: In:Rose, N. R., Friedmann, H. (eds.): Manual of clinical microbiology. American Society for Microbiology, Washington, D. C. 1976, pp. 506–512.

    Google Scholar 

  12. Svedhem, Å., Gunnarsson, H., Kaijser, B. Serological diagnosis ofCampylobacter jejuni infections by using the enzyme-linked immunosorbent assay principle. In:Newell, D. G. (ed.): Campylobacter. MTP Press Limited, Lancaster, England 1982, pp. 118–121.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bacteriology and Immunology, University of Helsinki, SF-00290, Helsinki 29

    T. U. Kosunen &  H. Rautelin

  2. Department of Medicine and Tropical Diseases, Aurora Municipal Hospital, SF-00250, Helsinki 25

    T. Pitkänen,  A. Pönka &  T. Pettersson

Authors
  1. T. U. Kosunen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Rautelin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Pitkänen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Pönka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Pettersson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kosunen, T.U., Rautelin, H., Pitkänen, T. et al. Antibodies against an acid extract from a single Campylobacter strain in hospitalized campylobacter patients. Infection 11, 189–191 (1983). https://doi.org/10.1007/BF01641193

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01641193

Keywords

Search

Navigation