Summary
By application of immunohistological methods in experimental alveolar echinococcosis, detailed information was obtained on the antigenic properties of each component of the echinococcus cyst. It was established that of the various layers, the capsula germinativa of the parasite cyst possesses the strongest antigenicity regardless of localization and maturity of the cysts. The dissemination of echinococcal antigens within the internal organs of the experimental animals was found to follow the general trends of other infections. Substances obtained from the capsula germinativa appear most suitable as reagents for immunodiagnostic purposes due to their strong antigenicity.
Zusammenfassung
Mit Hilfe immunhistologischer Methoden wurden bei der experimentellen alveolären Echinokokkose die Antigeneigenschaften verschiedener Schichten der Zysten untersucht. Es wurde festgestellt, daß die Capsula germinativa die höchste Antigenität unabhängig von der Lokalisation und der Zystenreife besitzt. Die Streuung des Echinokokkus-Antigens in die inneren Organe der Versuchstiere folgte der allgemeinen Richtung bei den Infektionskrankheiten. Die von Capsula germinativa gewonnenen Substanzen sind wegen ihrer hohen Antigenität am geeignesten als Reagenzien in der Immundiagnostik der Echinokokkose.
Article PDF
Similar content being viewed by others
Literature
Lamina, J. Zur Immundiagnostik menschlicher Helminthen-Infektionen. Münch. Med. Wschr. 116 (1974) 1466–1476.
Kagan, J. G. A review of serological tests for the diagnosis of hydatid disease. Bull. WHO 39 (1968) 25–37.
Huldt, G., Ljungström, I., Aust-Kettis, A. Detection by immunofluorescence of antibodies to parasitic agents. Use of class-specific conjugates. Ann. N. Y. Acad. Sci. 254 (1975) 304–314.
Gore, R. W., Sadun, E., Hoff, R. Echinococcus granulosus andE. multilocularis: Soluble antigen fluorescent antibody test. Exp. Parasitol. 28 (1970) 272–279.
Sadun, E. H. Seminar on immunity to parasitic helminths. VII. Fluorescent antibody technique for helminth infections. Exp. Parasitol. 13 (1963) 72–82.
Ambroise-Thomas, P.: Etude sero-immunologique de dix parasitose par la technique d'immunofluorescence. Thesis Lyon, 1969.
Dallenbach, F., Piekarski, G. On the demonstration of toxoplasma gondii in the tissue with the aid of labelled fluorescent antibodies (Coons method). Virchows Arch. (Pathol. Anat.) 333 (1960) 607–618.
Hinz, E., Kirsten, C. Fluoreszenzmikroskopische Antikörpertest bei experimenteller Echinokokkose. Diagnostik 5 (1972) 230.
Ruitenberg, E. J., Sleen, G. A modified immunofluorescence test for echinococcosis. J. Parasitol. 58 (1972) 347.
Hess, U., Eckert, J., Fröhlich, A. Vergleich serologischer Methoden für die Diagnose der zystischen und alveolären Echinokokkose des Menschen. Schweiz. Med. Wschr. 104 (1974) 853–859.
Coons, A., Kaplan, A. Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody. J. Exp. Med. 91 (1950) 1–13.
Silber, L.: Immunochemical Analysis. Medicina (Moscow) (1968) 6–20.
Hinz, E. Die Entwicklung des sekundärenEchinococcus multilocularis in experimentell infizierten Maus. Tropenmed. Parasit. 23 (1972) 256–265.
Lukaschenko, N.: Alveococcosis (alveolar echinococcosis). Medicina (Moscow) (1975) 47–68.
Swilenow, D., Heymer, B., Haferkamp, O. Rasterelektronenmikroskopische Untersuchungen der Cysten vonEchinococcus multilocularis. Z. Parasitenkd. 51 (1976) 25–29.
Biagi, F., Pina, A. Presence of antigens in calcareous corpuscles of cysticercus. Rev. Inst. Med. Trop. Sao Paulo 6 (1964) 114–116.
Svilenov, D., Heymer, B., Haferkamp, O.: Enzymhistochemische Untersuchungen der Leber bei der experimentellen alveolären Echinokokkose. Helminthologia (Sofia) (1977) in print.
Author information
Authors and Affiliations
Additional information
These investigations were supported by the World Health Organisation and the West German Federal Ministry for Youth, Family Affairs and Health.
Rights and permissions
About this article
Cite this article
Svilenov, D., Heymer, B. & Haferkamp, O. Immunomorphology and pathogenesis of echinococcosis. Infection 6, 12–15 (1978). https://doi.org/10.1007/BF01641084
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01641084