Summary
Lymphocytes from 22 children affected by chronic hepatitis were studied and a comparison was made with a control group of 32 healthy children in the same age range with respect to PHA dose-response curves, E-rosette formation and membrane immunoglobulin (Ig). From the results it appeared that PHA dose-response curves were displaced to the left (low PHA dose), DNA synthesis was significantly higher for all PHA dosages tested, and that the plasma of chronic hepatitis patients acted neither as inhibitor nor stimulator. The percentage of E-rosette-forming cells decreased, but the total number was normal. The mean percentage of Ig-bearing cells was normal but the single values were spaced over a wide range. It can be assumed that, at least in the chronic stage, there is an enhanced response to PHA and an increased percentage of cells without markers either for T or for B cells.
Zusammenfassung
Es wurden Lymphozyten von 22 mit chronischer Hepatitis befallenen Kindern mit 32 gesunden gleichaltrigen Kindern im Hinblick auf die DNA-Stimulierung, die E-Rosetten-Bildung und das Membran-Immunglobulin verglichen. Es ergab sich folgendes: Die Kurve der PHA-Stimulierung war nach links verschoben (niedrigere PHA-Dosis), die DNA-Synthese war bei den getesteten PHA-Dosierungen signifikant erhöht. Das Plasma von den Hepatitiskranken erwies sich weder als hemmend noch als stimulierend. Der Prozentsatz von E-Rosetten-bildenden Zellen war abgesunken, aber die Gesamtzellenzahl war normal. Die mittlere Prozentzahl von Ig-tragenden Zellen war normal, jedoch schwankten die einzelnen Werte in einem breiten Bereich. Es ist zu vermuten, wenigstens bei chronischen Erkrankungen, daß eine gesteigerte PHA-Reaktion auftritt, sowie eine prozentuale Zunahme von Zellen, die weder als B-noch als T-Zellen bei der Markierung in Erscheinung traten.
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Literatur
Tolentino, P. Chronische Virushepatitis im Kindesalter. Ein Beitrag mit 25 Fällen, davon 5 Geschwisterpaaren. Infection 1 (1973) 200.
Barker, L. L., Murray, R. Acquisitions of hepatitis-associated antigen. Clinical features in young adults. J. Amer. Med. Ass. 216 (1971) 1970.
Nielsen, J. O. et al. Incidence and Meaning of the “e” determinant among Australia Antigen-positive patients with acute and chronic liver diseases. Abstracts of the 9th Meeting of the E. A. S. L. Digestion 10 (1974) 304.
Dudley, F. J., Fox, R. A., Sherlock, S. Cellular immunity and hepatitis-associated, Australia antigen liver disease. Lancet I (1972) 723.
Giustino, V., Dudley, F. J., Sherlock, S. Thymus-dependent lymphocyte function in patients with hepatitis-associated antigen. Lancet II (1972) 850.
Sutnick, A. I., London, W. Th., Blumberg, B. S. Lymphocyte transformation and persistent Australia antigen. Lancet I (1973) 1124.
Clot, J., Mathieu, O., Elharrar, S., Robinet-Levy, M., Lemaire, T. Inhibitory effect of Australia-positive sera on in vitro-stimulated T lymphocytes. Lancet II (1973) 576.
Petri, I. B., Meszaros, E. J. Persistence of Au-SH antigen and lymphocyte transformation. Lancet II (1973) 802.
Braito, A., Giacchino, R. In vitro lymphocyte stimulation by PHA in HBAg-positive chronic hepatitis of children. Boll. Ist. Sieroter. Mil. 53 (1974) 64.
Böyum, A. Separation of lymphocytes and erythrocytes by centrifugation. Scand. J. Clin. Lab. Investig. 21 (1968) Suppl. 97 77.
Bloom, B. R., Landy, M., Lawrence, H. S. “In vitro„” methods in cell-mediated immunity: a progress report. Cellular Immunology 6 (1973) 331.
Hijmans, W., Schuit, H. R. E., Klein, F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin. Exp. Immunol. 4 (1969) 457.
Cochrane, M. A. G., Thomson, A. D., McFarlane, I. G., Eddleston, A. L. W. F., Williams, R. Mechanism of liver injury in chronic active hepatitis: Demonstration of lymphocyte cytotoxicity for isolated nepatocytes. Digestion 10 (1974) 329.
Paronetto, F., Vernace, S.: Immunological studies in patients with chronic active hepatitis. Cytotoxic activity of lymphocytes to autochthonous liver cells grown in tissue culture. Cl. Exp. Immun. (in press).
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Paper delivered at the Ninth Meeting of the European Association for the Study of the Liver (EASL), Hemsedal, Norway, 6 September 1974.
Study supported by Consiglio Nazionale delle Ricerche (Italian Research Council).
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Tolentino, P., Pasino, M., Braito, A. et al. Properties of lymphocytes in chronic HBAg-positive hepatitis. Infection 3, 78–82 (1975). https://doi.org/10.1007/BF01641046
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DOI: https://doi.org/10.1007/BF01641046