Skip to main content
SpringerLink
Log in

Beiträge zur Immundiagnostik der Filariosen bei Tropenrückkehrern sowie bei europäischen und autochthonen Bewohnern von Endemiegebieten

Immunological diagnosis of filariosis in persons returning from tropical countries and in European and indigenous inhabitants of endemic areas

  • Published:
Infection Aims and scope Submit manuscript

Zusammenfassung

Serumproben von Personen, die in Onchozerkosegebieten lebten oder eine Filariose hatten, wurden auf Antikörper gegen Rohextrakte aus Dirofilaria immitis, Onchocerca volvulus, Dipetalonema viteae und Ascaris suum in der Komplementbindung und indirekten Hämagglutination untersucht. Die Ergebnisse lassen folgende Deutungen zu: 1. Bei europäischen und autochthonen Bewohnern von Endemiegebieten mit und ohne Filariose erwies sich die indirekte Hämagglutination als der empfindlichere Test beim Nachweis von Antikörpern. 2. Bei 21 Loiasis-, 12 Onchozerkose-, 11 Dipetalonema perstans- und 22 Fällen klinischer Filariose ohne Mikrofilariennachweis konnten mit dem Serumverdünnungsfaktor 2 keine Titerdifferenzen gegen die vier Extrakte in beiden Tests festgestellt werden. Daraus wurde geschlossen, daß Untersuchungen mit Rohextrakten keine Aussagen über die Identität jener Antigene zulassen, die für die Stimulation der Antikörper verantwortlich waren.

Summary

Serum samples from persons who lived in areas where onchocerciasis occurred or who had filariasis were examined with the complement fixation test and the indirect hemagglutination test for the presence of antibodies against crude extracts from Dirofilaria immitis, Onchocerca volvulus, Dipetalonema viteae, and Ascaris suum. The results could be interpreted as follows: 1. The indirect hemagglutination test was more sensitive than the complement fixation test for the demonstration of antibodies in sera from European and indigenous inhabitants of endemic areas. 2. There were no differences between the responses to the four crude worm extracts among the groups of 21 patients with Loa loa, 12 patients with Onchocerca volvulus, 11 patients with Dipetalonema perstans, and 22 patients with clinical filariasis in whom no microfilaria had been found. It was concluded that the examination with crude extracts cannot give any information about the antigens that had caused the stimulation of antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  1. Ambroise-Thomas, P., Kien Truong, T. Application of the indirect fluorescent antibody test on sections of adult filariae to the serodiagnosis, epidemiology and posttherapeutic surveillance of human filariasis. Amer. Trop. Med. Parasit. 68 (1974) 435–452.

    Google Scholar 

  2. Ambroise-Thomas, P. Immunological diagnosis of human filariases: present possibilities, difficulties and limitations (a review). Acta tropica 31 (1974) 108–128.

    Google Scholar 

  3. Bagai, R. C., Subrahmanyam, D., Singh, V. B. Immunochemical studies with filarial antigens. Indian J. Med. Res. 56 (1969) 1064–1073.

    Google Scholar 

  4. Biguet, J., D'Haussy, R., Aubry, M., Rose, F. Étude des anticorps précipitants chez les sujets atteints d'onchocercose. Bull. Soc. Path. Exot. 57 (1964) 1098–1115.

    Google Scholar 

  5. Buck, A. A., Anderson, R. I., Kawata, K., Hitchcock, J. C. jr. Onchocerciasis: some new epidemiologic and clinical findings. Results of an epidemiologic study in the Republic of Chad. Amer. J. Trop. Med. Hyg. 18 (1969) 217–230.

    Google Scholar 

  6. Capron, A., Gentilini, M., Vernes, A. Le diagnostic immunologique des filarioses. Possibilités nouvelles offertes par l'immuno-électrophorèse. Path. Biol. 16 (1968) 1039–1045.

    Google Scholar 

  7. Colwell, E. J., Armstrong, D. R., Brown, J. D., Duxbury, R. E., Sadun, E. H., Legters, L. J. Epidemiologic and serologic investigations of filariasis in indigenous populations and american soldiers in South Vietnam. Am. J. Trop. Med. Hyg. 19 (1970) 227–231.

    Google Scholar 

  8. Frentzel-Beyme, R. The prevalence of onchocerciasis and blindness in the population of the Bong Range Liberia. Z. Tropenmed. Parasit. 24 (1973) 339–357.

    Google Scholar 

  9. Fujita, K. Cross-reaction among filarial species in hemagglutination test. Japan. J. Exp. Med. 40 (1970) 67–77.

    Google Scholar 

  10. Garms, R. Quantitative studies on the transmission of Onchocerca volvulus by Simulium damnosum in the Bong Range, Liberia. Z. Tropenmed. Parasit. 24 (1973) 358–372.

    Google Scholar 

  11. Gidel, R., Brengues, J., Rodhain, F. Essai de deux tests immunologiques (intradermoréaction et réaction de fixation du complément) pour le dépistage de filarioses dans des populations de Haute-Volta où coexistent Wuchereria bancrofti, Onchocerca volvulus et Dipetalonema perstans. Bull. Wld. Hlth. Org. 40 (1969) 831–842.

    Google Scholar 

  12. Kankonkar, S. Diagnosis of filariasis by indirect haemagglutination test. Indian J. Med. Res. 61 (1973) 1413–1415.

    Google Scholar 

  13. Marcoullis, G., Gräsbeck, R. Preliminary identification and characterization of antigen extracts from Onchocerca volvulus. Tropenmed. Parasit. 27 (1976) 314–322.

    Google Scholar 

  14. Neppert, J. Cross-reacting antigens among some filariae and other nematodes. Tropenmed. Parasit. 25 (1974) 454–463.

    Google Scholar 

  15. Neppert, J. Die Closed Hexagon Immundiffusion (CHI) in der Serodiagnostik der Onchozercose. Zbl. Bakt. Hyg. I. Abt. Orig. A 231 (1975) 297–300.

    Google Scholar 

  16. Niel, G., Gentilini, M., Courture, J., Pinon, J.-M., Danis, M. Application des extraits antigéniques d'Ascaris suum au diagnostic des filarioses en double diffusion. Valeur comparée à celle des antigènes Onchocerca volvulus et Dipetalonema viteae. Bull. Soc. Path. Exot. 65 (1972) 569–580.

    Google Scholar 

  17. Niel, G., Venard, P., Bruhnes, J., Charmot, G., Gentilini, M. La filariose de bancroft dans l'archipel des Comores (Étude immunologique). Bull. Soc. Path. Exot. 69 (1976) 85–92.

    Google Scholar 

  18. Ogunba, E. O. Serological investigations with Loa loa antigens. J. Helminth. 46 (1972) 241–250.

    Google Scholar 

  19. Pinon, J. M., Gentilini, M. Étude immunologique des filarioses humaines. Comparaison des tests cellulaires (rosettes inhibition de la migration des leucocytes) et des tests sérologiques. Nouv. Presse méd. 2 (1973) 1283–1287.

    Google Scholar 

  20. Schofield, F. D. The complement-fixation reaction in loiasis and Acanthocheilonema perstans infections. J. Trop. Med. Hyg. 60 (1957) 170–172.

    Google Scholar 

  21. Tanaka, H., Fujita, K., Sasa, M., Naito, M., Kurokawa, K. Cross-reactions in complement fixation test among filaria species. Japan. J. Exp. Med. 40 (1970) 47–58.

    Google Scholar 

  22. Weiss, N., Degrément, A. Vergleichende Untersuchungen zur Immundiagnostik der Filariasis. Tropenmed. Parasit. 27 (1976) 337–384.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bernhard-Nocht-Institut für Schiffs- und Tropenkrankheiten, Bernhard-Nocht-Str. 74, D-2000, Hamburg 4

    E. Mannweiler,  D. W. Büttner, I. Lederer & G. Dietz

  2. Oderfelder Str. 6, D-2000, Hamburg 13

    W. Mohr

  3. Tropenheim Paul-Lechler-Krankenhaus, D-7400, Tübingen

    W. Röllinghoff

Authors
  1. E. Mannweiler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. W. Büttner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Lederer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Dietz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. W. Mohr
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. W. Röllinghoff
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mannweiler, E., Büttner, D.W., Lederer, I. et al. Beiträge zur Immundiagnostik der Filariosen bei Tropenrückkehrern sowie bei europäischen und autochthonen Bewohnern von Endemiegebieten. Infection 5, 219–223 (1977). https://doi.org/10.1007/BF01640784

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01640784

Search

Navigation