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Recontamination after gastrointestinal decontamination with non-absorbable antibiotics

Rekontamination nach gastrointestinaler Dekontamination mit nichtresorbierbaren Antibiotika

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Summary

In an attempt to restore the colonization resistance we administered anaerobic microflora to prevent an abnormal colonization of the intestine after antibiotic treatment had been discontinued. After the antibiotics had been discontinued and before the donor flora had been administered and had colonized the intestine, microorganisms present were “unopposed” and expanded to a high density. A mouse model was used to investigate which antibiotics negatively influenced the donor flora and reduced the colonization resistance when administered intraperitoneally. Erythromycin, clindamycin and carbenicillin suppressed the donor flora permanently, as could be seen by the reduced colonization resistance. Benzylpenicillin, ampicillin, doxycycline and the combination gentamicin-cephalothin affected the colonization resistance as long as these agents were present. Gentamicin alone and cephalothin and oxytetracycline had no effect on the colonization resistance.

Zusammenfassung

Zur Wiederherstellung der Kolonisationsresistenz wurde anaerobe Mikroflora verabreicht, die die anormale Kolonisation des Darmes nach Unterbrechung der Antibiotikatherapie verhindern sollte. In dem Zeitraum zwischen Unterbrechung der Antibiotikagabe und der Verabreichung und dem Angehen der Spenderflora im Darm konnten sich bereits anwesende Mikroorganismen ungehindert in großer Dichte ausbreiten. In einem Mäusemodell wurde geprüft, welche Antibiotika bei intraperitonealer Gabe die Spenderflora negativ beeinflußten und die Kolonisationsresistenz verminderten. Erythromycin, Clindamycin und Carbenicillin führten zu einer anhaltenden Unterdrückung der Mikroflora, wie sich an der verminderten Kolonisationsresistenz ableiten ließ. Benzylpenicillin, Ampicillin, Doxycyclin und die Kombination Gentamicin-Cephalotin beeinträchtigten die Kolonisationsresistenz so lange wie diese Substanzen anwesend waren. Gentamicin allein und Cephalotin und Oxytetracyclin hatten keinen Einfluß auf die Kolonisationsresistenz.

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Authors and Affiliations

  1. CBL, Schiedamsedijk 95, 3011 EN, Rotterdam, The Netherlands

    W. D. H. Hendrik

  2. Radiobiological Institute TNO, 151 Lange Kleiweg, 2288 GJ, Rijswijk, The Netherlands

    J. de Vas

  3. Laboratory for Medical Microbiology, Oostersingel 59, 9713 EZ, Groningen, The Netherlands

    D. van der Waaij

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  1. W. D. H. Hendrik
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  2. J. de Vas
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  3. D. van der Waaij
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Hendrik, W.D.H., de Vas, J. & van der Waaij, D. Recontamination after gastrointestinal decontamination with non-absorbable antibiotics. Infection 9, 228–232 (1981). https://doi.org/10.1007/BF01640721

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  • DOI: https://doi.org/10.1007/BF01640721

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