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In-vitro-Aktivität des Oxa-Beta-Laktams LY 127935 gegenüber Bacteroides fragilis und anderen anaeroben gramnegativen Stäbchen

In vitro activity of the oxa-beta-lactam LY 127935 against bacteroides fragilis and other anaerobic gram-negative rods

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Zusammenfassung

Die Empfindlichkeit von 115Bacteroidaceae-Stämmen gegenüber LY 127935 wurde in Bouillondilutions-, Agardilutions- und Agardiffusions-versuchen bestimmt. Gleichzeitig wurden die Stämme auf Abbau des Oxa-Beta-Laktams untersucht. Nur fünf Stämme (2Bacteroides fragilis and 3Bacteroides oralis/Bacteroides bivius) bauten LY 127935 teilweise ab. Zwischen den Ergebnissen der Agardilutions- und Bouillondilutionsversuche wurden keine wesentlichen Abweichungen beobachtet. Nach den Ergebnissen der Agardilutionsmethode wurden 50B. fragilis-Stämme durch Konzentrationen von ≤8 µg Oxa-Beta-Laktam pro ml gehemmt. Angehörige derBacteroides melaninogenicus-Gruppe zeigten Agardilutions-MHK-Werte von ≤ 0,5 µg LY 127935 pro ml, undFusobacterium/Sphaerophorus-Arten wurden durch ≤64 µg/ml inhibiert. Stämme vonBacteroides thetaiotaomicron, B. oralis/B. bivius undBacteroides spp. waren weniger empfindlich. Für die Beziehung zwischen MHK und Hemmhofdurchmesser konnte wegen des engen Bereichs von MHK-Werten, der beiB. fragilis-Stämmen beobachtet wurde, keine Regressionsgerade berechnet werden.

Summary

The susceptibility of 115Bacteroidaceae strains to LY 127935 was determined by broth dilution, agar dilution and agar diffusion tests. Simultaneously, the strains were tested for degradation of the oxa-beta-lactam. Only five strains (2Bacteroides fragilis and 3Bacteroides oralis/Bacteroides bivius) partially degraded LY 127935. No major discrepancies between the results of the agar dilution and broth dilution tests were observed. According to the results obtained by the agar dilution method, 50 strains ofB. fragilis were inhibited by concentrations of ≤8 µg oxa-beta-lactam per ml. Members of theBacteroides melaninogenicus group of organisms had agar dilution MICs of ≤0.5 µg LY 127935 per ml andFusobacterium/Sphaerophorus species were inhibited by ≤64 µg/ml. Strains ofBacteroides thetaiotaomicron, B. oralis/B. bivius andBacteroides spp. were less susceptible. Due to the narrow range of MICs observed withB. fragilis strains, no regression line could be computed for the relationship between MIC and zone size.

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  1. Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Venusberg, D-5300, Bonn 1

    H. Werner &  C. Krasemann

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  1. H. Werner
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  2. C. Krasemann
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Werner, H., Krasemann, C. In-vitro-Aktivität des Oxa-Beta-Laktams LY 127935 gegenüber Bacteroides fragilis und anderen anaeroben gramnegativen Stäbchen. Infection 8 (Suppl 3), S342–S346 (1980). https://doi.org/10.1007/BF01639609

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