Summary
An in vitro study of the susceptibility of 201 newly isolated strains of gramnegative bacteria to six aminoglycoside antibiotics (kanamycin, amikacin, gentamicin, tobramycin, sisomicin and netilmicin) was performed by the twofold dilution method in fluid medium. Both the minimal inhibitory concentration and the minimal bacteridical concentration were determined. Overall, tobramycin seemed the most effective of the drugs studied. Netilmicin, the new derivative from sisomicin, compared favourably with the other drugs tested, but may, on theoretical grounds, offer the additional advantage of retained efficacy in the face of developing bacterial resistance. Not unexpectedly, amikacin appeared to be the most promising of the drugs studied in its action against Pseudomonas aeruginosa. Amikacin and netilmicin appeared to be the most effective of this group of antibiotics against Klebsiella species.
Zusammenfassung
Eine In-vitro-Studie über die Empfindlichkeit von 201 neu isolierten gramnegativen Bakterienstämmen gegenüber sechs Aminoglykosid-Antibiotika (Kanamycin, Amikacin, Gentamicin, Tobramycin, Sisomicin und Netilmicin) wurde mit Hilfe eines zweifachen Reihenverdünnungstests durchgeführt. Sowohl die minimale Hemmkonzentration als auch die minimale bakterizide Konzentration wurden bestimmt. Insgesamt erwies sich Tobramycin als das wirksamste der geprüften Medikamente. Im Vergleich zu den anderen untersuchten Medikamenten erzielte Netilmicin, das neue Derivat von Sisomicin, günstige Resultate. Außerdem hat dieses Medikament, rein theoretisch gesehen, den Vorteil einer stabileren Empfindlichkeit bei auftretender bakterieller Resistenz. Erwartungsgemäß erwies sich Amikacin als das zuverlässigste aller getesteten Medikamente gegen Pseudomonas aeruginosa. Als wirksamste Antibiotika gegen Klebsiella waren Amikacin und Netilmicin zu bezeichnen.
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Literature
Finland, M., Jones, W. F., Barnes, M. W. Change in the occurrence of serious bacterial infections since the introduction of antibacterial agents. Trans. Ass. Amer. Phys. 72 (1959) 305–321.
Yourassowsky, E., Schoutens, E., Vanderlinden, M. P. Comparison of the in vitro activities of BB-K8 and three other aminoglycosides against 215 strains of Pseudomonas and Enterobacteriaceae with variable sensitivity to kanamycin and gentamicin. Chemother. 21 (1975) 45–51.
Ximenes, J., Bassoi, O. N., de Meneres, J. P., Fry, W. Activity of amikacin, gentamicin and kanamycin against Pseudomonas aeruginosa. J. Int. Med. Res. 4 (1976) 165–175.
Kluge, R. M., Standiford, H. C., Tatem, B., Young, V. M., Greene, W. H., Schimpff, S. C., Calia, F. M., Hornick, R. B. Comparative activity of tobramycin, amikacin and gentamicin alone and with carbenicillin against Pseudomonas aeruginosa. Antimicr. Ag. Chemother. 6 (1974) 442–446.
Soussy, C. J., Duval, J. Activité antibactérienne comparée de sept aminosides. Méd. mal. infect. 6 (1976) 211–218.
Verbist, L., Spaepen, J., Vandepitte, J. In vitro sensitivity of hospital strains of Serratia marcescens to chemotherapeutic agents. Chemotherapy 22 (1976) 43–54.
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Although this article does not deal directly with antibiotic treatment, the article was accepted for publication because resistance to the aminoglycoside antibiotics is attracting increasing attention in antibacterial therapy in hospitals. This subject will be dealt with again in a coming issue of INFECTION in an article byH. Knothe andV. Krcméry entitled “Amikacin and Netilmicin Resistance in Pseudomonas aeruginosa.”
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Eyckmans, L., Beert, J. Sensitivity of gram-negative bacteria to six aminoglycoside antibiotics. Infection 5, 9–12 (1977). https://doi.org/10.1007/BF01639102
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DOI: https://doi.org/10.1007/BF01639102