Summary
A solid phase radioimmunoassay on microtiter plates was adapted for the estimation of mumps antibodies using commercially available complement-fixing mumps antigen. The sensitivity of the test is superior to that of hemagglutination inhibition and lies in the same range as the virus neutralization test performed on chick fibroblasts. The method is useful for screening large series of human sera, for instance in connection with vaccination programs.
Zusammenfassung
Eine radioimmunologische Bestimmung von Virusantikörpern mittels eines an Mikrotiterplatten fest gebundenen Antigens wurde entwickelt zur Bestimmung von Mumpsantikörpern. Als Antigen diente ein käufliches Komplementbindendes Mumps-Antigen. Die Methode ist empfindlicher als die Hämagglutinationshemmung; sie liegt im Bereich der Empfindlichkeit der Virusneutralisation auf Hühnerfibroblasten. Ihre Anwendung ist vor allem geeignet für große Serien von Antikörperbestimmungen in Humanseren, z. B. im Zusammenhang mit Impfprogrammen.
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Hernandez, R., Just, M. & Bürgin-Wolff, A. Screening for mumps immunity with the microtiter solid-phase radioimmunoassay. Infection 4, 121–124 (1976). https://doi.org/10.1007/BF01638936
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DOI: https://doi.org/10.1007/BF01638936