Hemoglobin function in stored blood

Summary

Inorganic phosphate which is known to stimulate red cell glycolysis is present in one of the preservatives for storing whole blood, citrate-phosphate-dextrose (CPD), but not the other, acid-citrate-dextrose (ACD). Both of these preservatives for liquid storage were developed before 2,3-diphosphoglycerate (2,3-DPG) was found to be necessary for normal hemoglobin function. In a recent study we have shown that very high concentrations of phosphate (10, 15, and 20 mM) were deleterious for maintaining 2,3-DPG. In the present study a lower range of phosphate concentrations (2, 4, 6, and 8 mM) was studied for maintenance of 2,3-DPG and ATP during storage under blood banking conditions. The lowest concentration, 2 mM, which corresponds to CPD was found to be the best concentration for maintaining 2,3-DPG and thus hemoglobin function. Four mM phosphate was not quite as good but better than no phosphate. Six and 8 mM phosphate were considerably worse.

Zusammenfassung

Im Anschluß an vorangegangene Arbeiten wurde die Anwendung von geringen Phosphatmengen (2–8 mM) zur Stabilisierung von 2,3-DPG und ATP während der Lagerung von Blut unter Blutbankbedingungen geprüft, um die Hämoglobinfunktion aufrecht zu erhalten. Am besten bewährte sich die 2mM-Konzentration des anorganischen Phosphats, während die 4mM-Konzentration weniger wirkungsvoll war. 6–8 mM-Konzentrationen wurden als ungeeignet befunden, die Stabilisierung von 2,3-DPG und ATP zu gewährleisten.