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Criteria for the differentiation of lymphoid cell lines

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Summary

Eleven lymphoid cell lines were initiated for culture simultaneously and sequentially from one healthy male donor and three from one female donor. There was no difference in respect to morphology (light- and electronmicroscopy) and growth characteristics. Chromosome pattern demonstrated tendency to aneuploidy except for one heteroploid cell line. Subterminal constrictions were found as B- and C-marker chromosomes in cell line II 1 and III 2. No other aberrations were typical for one cell line.Epstein-Barr Virus particles (EBV) were only seen in the oldest cell line. Seven cell lines were EBV-antigen positive, detected by immunofluorescent cell staining. The immunoglobulin pattern was constant. IgM was found in seven, IgG in three, and IgA in four cell lines. One cell line produced simultaneously IgM and IgG. It is concluded that cell lines are derived from B-type precursor cells and that they demonstrate clonal growth.

Zusammenfassung

Elf lymphoide Zellinien wurden gleichzeitig und nacheinander von einem gesunden männlichen und drei von einem weiblichen Spender zur Kultur angesetzt. Hinsichtlich der Morphologie (Licht- und Elektronenmikroskopie) und Wachstumseigenschaften gab es keinen Unterschied. Die chromosomalen Verteilungen zeigten eine Neigung zur Aneuploidie mit Ausnahme einer Zellinie, die heteroploid war. Subterminale Einschnürungen wurden als B und C gekennzeichnete Chromosomen in Zellinie II 1 und III 2 gefunden. Andere Abweichungen waren für keine Zellinie typisch.Epstein-Barr-Viruspartikel (EBV) wurden nur in der ältesten Zellinie gesehen. Mit der Immunofluoreszenz-Methode waren sieben Zellinien EBV-Antigen positiv. Das Immunglobulinmuster war konstant. IgM wurde in sieben, IgG in drei und IgA in vier Zellinien gefunden. Eine Zellinie produzierte gleichzeitig IgM und IgG. Es wird angenommen, daß die Zellinien von Vorläuferzellen des B-Typs abstammen und daß sie ein klonales Wachstum aufweisen.

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Author notes

  1. Hans Wulfard v. Heyden

    Present address: Section II Tübingen, Medical University Clinic, D-74, Tübingen, BRD

Authors and Affiliations

  1. Section II Tübingen, Medical University Clinic, D-74, Tübingen, BRD

    Dorit v. Heyden

  2. Department of Internal Medicine II, University of Tübingen, Tübingen, Germany

    Hans Wulfard v. Heyden & Dorit v. Heyden

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  1. Hans Wulfard v. Heyden
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  2. Dorit v. Heyden
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This work was supported by the “Deutsche Forschungsgemeinschaft”, Bonn-Bad Godesberg.

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v. Heyden, H.W., v. Heyden, D. Criteria for the differentiation of lymphoid cell lines. Blut 29, 323–331 (1974). https://doi.org/10.1007/BF01634017

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  • DOI: https://doi.org/10.1007/BF01634017

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