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Evidence of platelet complement-fixing and lymphocytotoxic anti-A1 intibodies

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Summary

3 human antisera exhibiting non HL-A specific antibody specificity were studied in micro-complement fixation with platelets and micro-lymphocytotoxicity. Screening studies as well as absorption and elution studies with platelets, lymphocytes and erythrocytes revealed an antibody activity associated with blood group A1.

Within blood group A1, a subpopulation of A1 donor cells-provisionally called “weak A1”-was defined, which had a minor ability for complement-fixing and lymphocytotoxic A1-antibody absorption.

Contrary to HL-A specific antibodies, acid eluates from platelets of these 3 sera were always negative whereas heat and ether eluated gave positive results.

From neutralization studies and studies on the hemolytic activity against A1 erythrocytes it was concluded, that the a1-specific antibodies probaly are immune antibodies belonging to the IgG class of immunoglobulins.

Zusammenfassung

3 humane Antiseren, die von HL-A-spezifischen Antikörpern abzugrenzende Antikörperspezifitäten enthielten, wurden in der plättchen-Mikro-KLomplementbindungsreaktion und der Mikro-Lymphozytotoxizitätsreaktion untersucht.

Reihenuntersuchungen sowie Absorptions- und Elutgionsuntersuchungen mit Plättchen, Lymphozyten und Erythrozyten ergaben eine Korrelation der Antikörperaktivität mit der Blutgruppe A1.

Innerhalb der Blutgruppe A1 wurde eine Subpopulation von A1-Spendern-vorläufig “schwach A1” genannt-gefunden, deren Zellen eine verminderte Komplementfixation und eine verminderte Absorption von lymphozytotoxische A1-Antikörpern aufwies.

Im Gegensatz zu HL-A-spezifischen Antikörpern waren die Säureeluate der 3 Antiseren mit Plättchen stets negativ, Wärme- und Äthereluate dagegen positiv.

Untersuchungen über die Neutralisation dieser Antikörper und Untersuchungen über ihre hämolytische Aktivität gegen A1-Erythrozyten führten zu der Schlui\folgerung, daß die A1-spezifischen Antikörper sehr wahrscheinlich Immunantikörper sind und zur IgG-Immunglobulinklasse gehören.

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Author notes

  1. Christian Mueller-Eckhardt

    Present address: Institut of Clinical Immunology and Blood Transfusion, University of Gießen, Langhansstr. 7, D-63, Gie\en, Germany

Authors and Affiliations

  1. Institut of Clinical Immunology and Blood Transfusion, University of Gießen, Langhansstr. 7, D-63, Gie\en, Germany

    Wolfgang Gross, Dieter Heinrich, Peter Walker & Karin Mersch-Baumert

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  1. Wolfgang Gross
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  2. Dieter Heinrich
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  3. Peter Walker
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  4. Karin Mersch-Baumert
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  5. Christian Mueller-Eckhardt
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Supported by the Deutsche Forschungsgemeinschaft (Mu 277.5)

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Gross, W., Heinrich, D., Walker, P. et al. Evidence of platelet complement-fixing and lymphocytotoxic anti-A1 intibodies. Blut 30, 101–106 (1975). https://doi.org/10.1007/BF01633964

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