Skip to main content

Advertisement

SpringerLink
Log in

Production of antibodies specific for human thymus derived lymphocytes purified from antibodies crossreacting with colony-forming cells

  • Kurze Mitteilung
  • Published:
Blut: Zeitschrift für die Gesamte Blutforschung Aims and scope Submit manuscript

Summary

Antisera raised in rabbits against human thymocytes contain antibodies against peripheral lymphocytes of normal persons, patients with chronic lymphatic leukemia (CLL) and lymphoblastoid cell lines. Absorption of anti-thymocyte serum with CLL-lympphocytes and spleen preserved the antibody activity against normal peripheral lymphocytes and a lymphoblastoid cell line of T-cell type (MOLT-4), while eliminating antibodies reacting with CLL-lymphocytes and a B-cell type lymphoblastoid cell line (RPMI 1788). The incubation of human bone marrow cells with not absorbed anti-thymocyte globulin reduced considerably the number of stem cells committed to granulopoietic differentiation (CFU-C). No reduction of CFU-C, however, was observed with specifically absorbed anti-thymocyte globulin. The possible application of stem-cell-sparing, purified anti-thymocyte globulin for the suppression of graft-versus-host reactions in human bone marrow transplantation is discussed.

Zusammenfassung

In Kaninchen produzierte, gegen menschliche Thymozyten gerichtete Antiseren enthalten Antikörper gegen periphere Lymphozyten von Normalpersonen und Patienten mit chronisch-lymphatischer Leukämie (CLL) sowie gegen lymphoblastoide Zelllinien. Eine Absorption von Anti-Thymozyten-Serum mit CLL-Lymphozyten und Milz führt zu Antikörperpräparationen, die mit normalen peripheren Lymphozyten und einer lymphoblastoiden Zellinie vom T-Zell-Typ reagieren, aber keine Aktivität gegen CLL-Lymphozyten oder eine lymphoblastoide Zellinie vom B-Zell-Typ mehr zeigen.

Während die Inkubation von menschlichem Knochenmark mit nicht absorbiertem Anti-Thymozyten-Globulin die Zahl der granulozytopoetisch determinierten Stammzellen (CFU-C) erheblich reduziert, wurde nach Inkubation mit spezifisch absorbiertem Anti-Thymozyten-Globulin keine Verminderung der CFU-C gefunden. Die mögliche Verwendung von stammzellschonendem, gereinigtem Anti-Thymozyten-Globulin zur Unterdrückung der Spender-gegen-Wirt-Reaktion im Rahmen der Knochenmarktransplantation wird diskutiert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bobrove, A. M., S. Strober, L. A. Herzenberg and J. D. de Pamphilis: Identification and quantification of thymus-derived lymphocytes in human peripheral blood. J. Immunol.112, 520 (1974).

    CAS  PubMed  Google Scholar 

  2. Brown, G. and M. F. Greaves: Cell surface markers for human T and B lymphocytes. Europ. J. Immunol.4, 302 (1974).

    Article  CAS  Google Scholar 

  3. Brown, G. and M. F. Greaves: Enumeration of absolute numbers of T and B lymphocytes in human blood. Scand. J. Immunol.3, 161 (1974).

    Article  CAS  PubMed  Google Scholar 

  4. Dicke, K. A., M. J. van Noord, B. Maat, U. W. Schaefer and D. W. van Bekkum: Identification of cells in primate bone marrow resembling the hemapoietic stem cell in the mouse. Blood42, 195 (1973).

    CAS  PubMed  Google Scholar 

  5. Fröland, S. S.: Binding of sheep erythrocytes to human lymphocytes. A probable marker of T lymphocytes. Scand. J. Immunol.6, 269 (1972).

    Article  Google Scholar 

  6. Golub, E. S.: Brain-associated θ antigen: Reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell. Immunol.2, 353 (1971).

    Article  CAS  PubMed  Google Scholar 

  7. Han, T., J. L. Pauly and J. Minowada: In vitro preferential effect of irradiation on cultured T lymphoid cell line. Clin. exp. Immunol.17, 455 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Minowada, J., T. Ohnuma and G. E. Moore: Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Nat. Cancer Inst.49, 891 (1972).

    CAS  PubMed  Google Scholar 

  9. Pike, B. L. and W. A. Robinson: Human bone marrow colony growth in agar-gel. J. Cell. Physiol.76, 77 (1970).

    Article  CAS  PubMed  Google Scholar 

  10. Reif, A. E. and I. M. V. Allen: The AKR thymic antigen and its distribution in leukemias and nervous tissue. J. exp. Med.120, 413 (1964).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Ringelmann, R., W. Opferkuch, M. Röllinghoff und M. Loos Komplementrnessungen mit Hilfe des Mikrolitersystems. Z. Med. Mikrobiol. Immunol.154, 329 (1969).

    Article  CAS  PubMed  Google Scholar 

  12. Rodt, H., S. Thierfelder and M. Eulitz: Antilymphocytic antibodies and marrow transplantation. III. Effect of heterologous anti-brain antibodies on acute secondary disease in mice. Europ. J. Immunol.4, 25 (1974).

    Article  Google Scholar 

  13. Rodt, H., S. Thierfelder and M. Eulitz: Suppression of acute secondary disease by heterologous anti-brain serum. Blut25, 385 (1972).

    Article  CAS  PubMed  Google Scholar 

  14. Shevach, E. M., R. Herbermann, M. M. Frank and I. Green: Receptors for complement and immunoglobulin on human leukemic cells and human lymphoblastoid cell lines. J. Clin. Invest.51, 1933 (1972).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Smith, R. W., W. D. Terry, D. N. Buell and K. W. Sell: An antigenic marker for human thymic lymphocytes. J. Immunol.110, 884 (1973).

    CAS  PubMed  Google Scholar 

  16. Thiele, H.-G., R. Stark and D. Keeser: Antigenic correlations between brain and thymus. I. Common antigenic structures in rat and mouse brain tissue and thymocytes. Europ. J. Immunol.2, 424 (1972).

    Article  CAS  Google Scholar 

  17. Touraine, J. L., F. Touraine, D. F. Kiszkiss, Y. S. Choi and R. A. Good: Heterologous specific antiserum for identification of human T lymphocytes. Clin. Exp. Immunol.16, 503 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Williams, R. C., J. R. de Board, O. J. Mellbye, R. P. Messner and F. D. Lindström: Studies of T- and B-Lymphocytes in Patients with Connective Tissue Diseases. J. Clin. Invest.52, 283 (1973).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Author notes

  1. Hans Rodt

    Present address: Abteilung Immunologie, Institut für Hämatologie der Gesellschaft für Strahlen- und Umweltforschung mbH, Landwehrstraße 61, D-8000, München 2, BRD

Authors and Affiliations

  1. Abteilung Immunologie, Institut für Hämatologie der Gesellschaft für Strahlen- und Umweltforschung mbH, Landwehrstraße 61, D-8000, München 2, BRD

    Bruno Netzel, Gerd Brehm & Stefan Thierfelder

Authors
  1. Hans Rodt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bruno Netzel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gerd Brehm
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stefan Thierfelder
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This study was supported by the Association EURATOM-GSF Hämatologie No. 031-64 I BIAD and the SFB 37.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodt, H., Netzel, B., Brehm, G. et al. Production of antibodies specific for human thymus derived lymphocytes purified from antibodies crossreacting with colony-forming cells. Blut 29, 416–421 (1974). https://doi.org/10.1007/BF01633677

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01633677

Keywords

Search

Navigation