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Vergleichende Untersuchung verschiedener Methoden zur Isolierung peripherer menschlicher Lymphozyten

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Zusammenfassung

Beim Vergleich verschiedener Methoden, die der Isolierung von Lymphozyten aus dem menschlichen Blut dienten, hat sich die Säulentrennung in bezug auf die Reinheit der Lymphozytensuspensionen als die beste Methode erwiesen. Die Trennungen der Blutzellen auf Grund ihrer unterschiedlichen Dichte führte bei besseren Zellausbeuten zu Zellsuspensionen mit großem Monozytenanteil.

Summary

Comparing various methods for separation of human peripheral lymphocytes from other blood cells purification on nylon fibre columns has proved to be the best one. With other methods basing on different cell density higher numbers of cells were obtained, but cell suspensions were markedly contaminated with monocytes.

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Author notes

  1. H. Scholle, W. Schlag &  H. Rühl

    Present address: Hämatologischen Abteilung, Klinikum Steglitz der Freien Universität Berlin, Hindenburgdamm 30, D-1, Berlin 45

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    1. H. Scholle
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    2. W. Schlag
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    3. H. Rühl
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    Scholle, H., Schlag, W. & Rühl, H. Vergleichende Untersuchung verschiedener Methoden zur Isolierung peripherer menschlicher Lymphozyten. Blut 24, 54–57 (1972). https://doi.org/10.1007/BF01633142

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    • DOI: https://doi.org/10.1007/BF01633142

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