, Volume 112, Issue 3, pp 117–124 | Cite as

Microtubule- and microfilament-based dynamic activities of the endoplasmic reticulum and the cell surface in epithelial cells ofSpongilla lacustris (Porifera, Spongillidae)

  • Dieter Wachtmann
  • Wilhelm Stockem


Dynamic activities of the endoplasmic reticulum (ER) and of the cell surface were analyzed in living epithelial cells (pinacocytes) ofSpongilla lacustris by contrast-enhanced video microscopy with the AVEC-DIC or the ACE equipment. Long term sequences revealed the ER to be a highly unstable system undergoing permanent alterations of the reticular patterns in that tubules merge and split or polygons open and close again. Treatment with colcemid or colchicine causes distinct changes of the typical motile phenomena, whereas cytochalasin D has no influence. On the other hand, the dynamic behavior of the cell surface is characterized by distinct ruffling activities as well as the formation and retraction of spiky filopodia. In contrast to the described ER dynamics, cell surface phenomena are clearly influenced by cytochalasin D but not by colcemid or colchicine. Altogether, results of the present paper are similar to correspondent observations on mammalian cells and point to microtubules and microfilaments as the cytoskeletal elements being responsible for ER and cell surface dynamics, respectively.


Cell Surface Endoplasmic Reticulum Colchicine Cytochalasin Dynamic Activity 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Dieter Wachtmann
    • 1
  • Wilhelm Stockem
    • 1
  1. 1.Institute of Cell BiologyUniversity of BonnBonn 1Germany

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