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The calibration by electronic means of Coulter counter for the determination of absolute volume of particles

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Summary

An electronic method is described for the calibration of a particle sizing apparatus based on the principle employed by Coulter counters, without any need for reference particles. A capillary factor, depending upon the dimensions of the orifice, has been obtained by reference measurements. Incorporating this capillary factor and the particle form factor, the absolute particle volumes can be deduced.

Zusammenfassung

Die Arbeit beschreibt eine elektronische Methode zur Eichung von Partikelvolumen-Meßgeräten nach dem Coulter-Prinzip ohne Verwendung von Referenzpartikeln bekannter Größe. Durch Referenzmessungen wird ein von den Dimensionen der Meßöffnung abhängiger Kapillarfaktor bestimmt. Die Einbeziehung dieses Kapillarfaktors in bekannte Berechnungsformeln gestattet bei Kenntnis des Partikelformfaktors eine Berechnung absoluter Volumina.

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Author notes

  1. Helmut Metzger,  Günter Valet,  Volker Kachel &  Gerhard Ruhenstroth-Bauer

    Present address: Max-Planck-Institut für Biochemie, 8533, Martinsried bei München

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    1. Helmut Metzger
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    2. Günter Valet
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    3. Volker Kachel
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    4. Gerhard Ruhenstroth-Bauer
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    Additional information

    This work has been supported by a grant from the Ministerium für Bildung und Wissenschaft der Bundesrepublik Deutschland

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    Metzger, H., Valet, G., Kachel, V. et al. The calibration by electronic means of Coulter counter for the determination of absolute volume of particles. Blut 25, 179–184 (1972). https://doi.org/10.1007/BF01632390

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    • DOI: https://doi.org/10.1007/BF01632390

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