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Dipeptidyl peptidase IV, prolyl endopeptidase and cathepsin B activities in primary human lung tumours and lung parenchyma

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  • Experimental Oncology
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Summary

The activities of dipeptidyl peptidase IV (DPP-IV), prolyl endopeptidase (PE) and cathepsin B (CB) were investigated in primary human lung tumours and matched lung parenchyma, using continuous-rate fluorometric assays of the enzymes. Squamous-cell lung carcinomas showed significantly higher specific activities of all three enzymes studied. In lung adenocarcinomas, only activities of PE and CB were increased significantly. In a limited number of primary human lung tumours of other histological types the activities of DPP-IV, PE and CB were also elevated. Mixing the matched homogenates of lung tumours and lung parenchyma gave additive activities for each enzyme studied. A significant correlation between tumour/lung ratios of specific activities of DPP-IV and CB was observed.

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Abbreviations

DPP-IV:

dipeptidyl peptidase IV

PE:

prolyl endopeptidase

CB:

cathepsin B

Ac-Leu-Arg-Arg-NHMec:

7-(N- acetyll-leucyl-l-arginyl-l-arginylamido)-4-methylcoumarin

Gly-Pro-NHMec:

7-(glycyl-l-prolylamido)-4-methylcoumarin

Suc-Gly-Pro-NHMec:

7-(N-succinylglycyl-l-prolylamido)-4-methylcoumarin

NH2Mec:

7-amino-4-methylcoumarin

r :

Pearson's correlation coefficient

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šedo, A., KŘepela, E. & Kasafírek, E. Dipeptidyl peptidase IV, prolyl endopeptidase and cathepsin B activities in primary human lung tumours and lung parenchyma. J Cancer Res Clin Oncol 117, 249–253 (1991). https://doi.org/10.1007/BF01625433

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  • DOI: https://doi.org/10.1007/BF01625433

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