Skip to main content
Log in

Dipeptidyl peptidase IV, prolyl endopeptidase and cathepsin B activities in primary human lung tumours and lung parenchyma

  • Original Papers
  • Experimental Oncology
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript


The activities of dipeptidyl peptidase IV (DPP-IV), prolyl endopeptidase (PE) and cathepsin B (CB) were investigated in primary human lung tumours and matched lung parenchyma, using continuous-rate fluorometric assays of the enzymes. Squamous-cell lung carcinomas showed significantly higher specific activities of all three enzymes studied. In lung adenocarcinomas, only activities of PE and CB were increased significantly. In a limited number of primary human lung tumours of other histological types the activities of DPP-IV, PE and CB were also elevated. Mixing the matched homogenates of lung tumours and lung parenchyma gave additive activities for each enzyme studied. A significant correlation between tumour/lung ratios of specific activities of DPP-IV and CB was observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others



dipeptidyl peptidase IV


prolyl endopeptidase


cathepsin B


7-(N- acetyll-leucyl-l-arginyl-l-arginylamido)-4-methylcoumarin







r :

Pearson's correlation coefficient


  • Brunk CF, Jones K, James TW (1979) Assay for nanogram quantities of DNA in cellular homogenates. Anal Biochem 92:497–500

    Google Scholar 

  • Burnett D, Crocker J, Stockley RA (1983) Cathepsin B-like cysteine proteinase activity in sputum and immunohistological identification of cathepsin B in alveolar macrophages. Am Rev Respir Dis 128:915–919

    Google Scholar 

  • Chapman HA Jr, Reilly JJ Jr, Yee R, Grubb A (1990) Identification of cystatin C, a cysteine proteinase inhibitor, as a major secretory product of human alveolar macrophages in vitro. Am Rev Respir Dis 141:698–705

    Google Scholar 

  • Chung SM, Kawai K (1990) Protease activities in gastric cancer tissues. Clin Chim Acta 189:205–210

    Google Scholar 

  • Collier IE, Wilhelm SM, Eisen AZ, Marmer BL, Grant GA, Seltzer JL Kronberger A, He C, Bauer EA, Goldberg GI (1988) H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloproteinase capable of degrading basement membrane collagen. J Biol Chem 263:6579–6587

    Google Scholar 

  • Downs TR, Wilfinger WW (1983) Fluorometric quantification of DNA in cells and tissue. Anal Biochem 131:538–547

    Google Scholar 

  • Erdel M, Trefz G, Spiess E, Habermaas S, Spring H, Lah T, Ebert W (1990) Localization of cathepsin B in two human lung cancer cell lines. J Histochem Cytochem 38:1313–1321

    Google Scholar 

  • Gottesman MM (1990) Introduction: do proteases play a role in cancer? Semin Cancer Biol 1:97–98

    Google Scholar 

  • Howie AJ, Burnett D, Crocker J (1985) The distribution of cathepsin B in human tissues. J Pathol 145:307–314

    Google Scholar 

  • Kato T, Nagatsu TT, Kimura T, Sakakibara S (1978) Fluorescence assay of X-prolyl dipeptidyl-aminopeptidase activity with a new fluorogenic substrate. Biochem Med 19:351–359

    Google Scholar 

  • Kato T, Okada M, Nagatsu T (1980) Distribution of post-proline cleaving enzyme in human brain and the peripheral tissues. Mol Cell Biochem 32:117–121

    Google Scholar 

  • Keski-Oja J, Blasi F, Leof EB, Moses HL (1988) Regulation of the synthesis and activity of urokinase plasminogen activator in A 549 human lung carcinoma cells by transforming growth factor-Β. J Cell Biol 106:451–459

    Google Scholar 

  • KŘepela E, Vičar J, ŽiŽková L, Kasafírek E, KoláŘ Z, Lichnovský V (1985) Dipeptidyl peptidase IV in mammalian lungs. Lung 163:33–54

    Google Scholar 

  • KŘepela E, Kasafírek E, Novák K, Viklický J (1990) Increased cathepsin B activity in human lung tumors. Neoplasma 37:61–70

    Google Scholar 

  • Kubochi K, Otani Y, Maruyama K, Okazaki I (1986) Development of a direct measurement assay for collagenase against type I and type IV collagens in tissue homogenate and its application in stomach and lung cancers. In: Tschesche H (ed) Proteinases in inflammation and tumor invasion. de Gruyter, Berlin New York, pp 337–356

    Google Scholar 

  • Kyllonen AP, Jarvinen M, Hopsu-Havu VK, Dorn A, Rasanen O, Larmi T, Rinne A (1984) Das Verhalten der kleinmolekularen Cysteinproteinase-Inhibitoren in Lungencarcinomen und den sie umgebenden Geweben. Acta Histochem 74:109–113

    Google Scholar 

  • Markus G, Takita H, Camiolo SM, Corasanti JG, Evers JL, Hobika GH (1980) Content and characterization of plasminogen activators in human lung tumors and normal lung tissue. Cancer Res 40:841–848

    Google Scholar 

  • Momiki S, Caamano J, Sickler A, Ruggeri B, Klein-Szanto AJP (1990) Type IV collagenase activity in human lung tumor cells correlates with their invasive and metastatic potential. Proc Am Assoc Cancer Res 31:73

    Google Scholar 

  • Mountain CF (1987) The new international staging system for lung cancer. Surg Clin N Am 67:925–935

    Google Scholar 

  • Reid WA, Valler MJ, Kay J (1986) Immunolocalisation of cathepsin D in normal and neoplastic human tissues. J Clin Pathol 39:1323–1330

    Google Scholar 

  • šedo A, Adamec M (1990) A Pascal program for computing DNA concentrations in cellular homogenates. Comput Methods Programs Biomed 33:189–190

    Google Scholar 

  • šedo A, KŘepala E, Kasafírek E (1989) A kinetic fluorometric assay of dipeptidyl peptidase IV in viable human blood mononuclear cells. Biochimie 71:757–761

    Google Scholar 

  • Taylor GM, Cook HT, Sheffield EA, Hanson C, Peart WS (1988) Renin in blood vessels in human pulmonary tumors. An immunohistochemical and biochemical study. Am J Pathol 130:543–551

    Google Scholar 

  • Trefz G, Lüthgens K, Erdel M, Spiess E, Ebert W (1989) Plasminogen activator and cathepsin B in normal and malignant human lung tissue. J Cancer Res Clin Oncol 115:S50

    Google Scholar 

  • Trefz G, Erdel M, Spiess E, Ebert W (1990) Detection of cathepsin B, plasminogen activators and plasminogen activator inhibitor in human non-small lung cancer cell lines. Biol Chem Hoppe-Seyler 371:617–624

    Google Scholar 

  • Ura H, Bonfil RD, Reich R, Reddel R, Pfeifer A, Harris CC (1989) Expression of type IV collagenase and procollagen genes and its correlation with the tumorigenic, invasive and metastatic abilities of oncogene-transformed human bronchial epithelial cells. Cancer Res 49:4615–4621

    Google Scholar 

  • Walter R, Simmons WH, Yoshimoto T (1980) Proline specific endo- and exopeptidases. Mol Cell Biochem 30:111–127

    Google Scholar 

  • World Health Organization (1981) Histological typing of lung tumors 2nd international histological classification of tumors: no. 1. WHO, Geneva

    Google Scholar 

  • Yoshii Y, Kasugai T, Kato T, Nagatsu T, Sakakibara S (1981) Changes in serum dipeptidyl-aminopeptidase IV (glycylprolyl dipeptidylaminopeptidase) activity of patients with gastric carcinoma after surgical excision and the enzyme activity in carcinoma tissue. Biochem Med 25:276–282

    Google Scholar 

  • Yoshimoto T, Tsukumo K, Takatsuka N, Tsuru D (1982) An inhibitor for post-proline cleaving enzyme: distribution and partial purification from porcine pancreas. J Pharmacobiodyn 5:734–740

    Google Scholar 

  • Zacharski LR, Memoli VA, Rousseau SM, Kisiel W (1987) Occurrence of blood coagulation factors in situ in small cell carcinoma of the lung. Cancer 60:2675–2681

    Google Scholar 

  • Zolfaghari R, Baker CRF, Canizaro PC, Feola M, Amirgholami A, BĚhal FJ (1986) Human lung post-proline endopeptidase: purification and action on vasoactive peptides. Enzyme 36:165–178

    Google Scholar 

  • Zucker S, Wieman JM, Lysik RM, Wilkie D, Ramamurthy NS, Golub LM, Lane B (1987) Enrichment of collagen and gelatin degrading activities in the plasma membranes of human cancer cells. Cancer Res 47:1608–1614

    Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

šedo, A., KŘepela, E. & Kasafírek, E. Dipeptidyl peptidase IV, prolyl endopeptidase and cathepsin B activities in primary human lung tumours and lung parenchyma. J Cancer Res Clin Oncol 117, 249–253 (1991).

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI:

Key words