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Circadian anorectic effects of peripherally administered amylin in rats

Zirkadiane anorektische Effekte von peripher verabreichtem Amylin bei Ratten

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Summary

The pancreatic peptide amylin (1 µg/kg) injected intraperitoneally reduced cumulative food intake for up to 4 h in food-deprived (24 h) and non-deprived rats at various times of the day, i.e., at dark onset, in the middle of the dark phase, and at light onset. At none of these times did subdiaphragmatic vagotomy abolish the anorectic effect of amylin. Rather, vagotomy enhanced, by unknown mechanisms, amylin's anorectic effect in food-deprived rats at light onset and in the middle of the dark phase. In contrast to previous studies with older rats, amylin's anorectic effect was also observed when injected into nondeprived rats. The findings of the present study extend previous reports in that amylin's anorectic effect, not being abolished by abdominal vagotomy after intraperitoneal injection, can be elicited at different times of the day.

Zusammenfassung

Das aus dem Pankreas stammende Peptid Amylin (1 µg/kg) reduzierte nach intraperitonealer (IP) Injektion die kumulative Futteraufnahme bei Ratten, denen vor der Injektion das Futter für 24 h entzogen worden war bzw. bei Ratten, denen Futter ad lib. zur Verfügung stand, für bis zu 4 h. Der Effekt trat zu verschiedenen Zeitpunkten des Tag/Nacht-Zyklus (Beginn der Dunkelphase, Mitte der Dunkelphase, Beginn der Hellphase) auf. Der verzehrsreduzierende Effekt von IP verabreichtem Amylin wurde zu keinem dieser Zeitpunkte durch subdiaphragmatische Vagotomie aufgehoben. Vagotomie führte eher zu einer Verstärkung des verzehrsreduzierenden Effekts von Amylin, und zwar nach einem 24stündigen Futterentzug bei Injektion zu Beginn der Hellphase oder in der Mitte der Dunkelphase. Im Unterschied zu früheren Untersuchungen mit älteren Ratten konnte ein verzehrsreduzierender Effekt von Amylin auch bei Ratten beobachtet werden, denen vor der Injektion das Futter nicht entzogen worden war.

In Ergänzung früherer Befunde zeigen also die Ergebnisse, daß der verzehrsreduzierende Effekt von IP verabreichtem Amylin zu verschiedenen Tageszeiten ausgelöst und durch subdiaphragmatische Vagotomie nicht aufgehoben werden kann.

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Abbreviations

CGRP:

calcitonin gene-related peptide

IP :

intraperitoneal

SVAG :

sham vegotomized

VAG :

vagotomized

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  1. Institute für Veterinär-Physiologie, Winterthurer Straße 260, 8057, Zürich, Schweiz

    T. A. Lutz, E. Del Prete, M. M. Szabady & E. Scharrer

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  1. T. A. Lutz
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  2. E. Del Prete
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  3. M. M. Szabady
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  4. E. Scharrer
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Lutz, T.A., Del Prete, E., Szabady, M.M. et al. Circadian anorectic effects of peripherally administered amylin in rats. Z Ernährungswiss 34, 214–219 (1995). https://doi.org/10.1007/BF01623160

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  • DOI: https://doi.org/10.1007/BF01623160

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