Summary
If phosphate buffer of the same pH as serum (about 7.6) is added to a mixture of taurocholate and red cells, inhibition or acceleration of lysis is observed according to whether the lysin and the cells have reacted for a short or a long time. If serum or plasma is added under the same conditions, the inhibition and acceleration are very much greater.Sen's conclusion that the pH of the serum must be partly responsible for these effects is therefore confirmed, but the effects attributable to pH change are subsidiary ones.
Dilution of a cell-taurocholate system with saline may give rise to inhibition and acceleration phenomena, and the origin of these is discussed.
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References
Ponder, E., 1922. Proc. Roy. Soc., B,93, 86.
—, 1923. Proc. Roy. Soc., B,95, 382.
—, 1924. Biochem. J.,18, 845.
— andA. S. Gordon, 1935. Proc. Roy. Soc, B,117, 272.
Schulman, J. H. andE. K. Rideal, 1937. Proc. Roy. Soc, B,122, 46.
Sen, K. C., 1931. Biochem. J.,25, 1244.
— andN. N. Mirka, 1928. J. Ind. Chem. Soc.,5, 683.
— andA. C. Roy, 1930–31. Ind. J. Med. Research,18, 881.
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Ponder, E. pH as a factor in producing acceleration in cell-taurocholate-serum systems. Protoplasma 30, 577–581 (1938). https://doi.org/10.1007/BF01613842
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DOI: https://doi.org/10.1007/BF01613842