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Protoplasma

, Volume 30, Issue 1, pp 101–103 | Cite as

A new method for the detection of carotenoids in chlorophyll samples

  • B. N. Singh
  • N. K. Anantha Rao
Abhandlungen

Summary

A new method is developed for the detection of carotenoids in chlorophyll samples.

The typical colour curve of chlorophyll in 80 % methyl alcohol exhibits marked absorption in 690μμ to 610μμ and less absorption in 500μμ to 430μμ. The colour curve of chlorophyll contaminated with carotenoids exhibits higher values in the region 530μμ to 430μμ. If carotin is present, the band maximum in the region 530μμ to 430μμ is located at 500μμ, and if xanthophyll is the impurity the band maximum is shifted to 430μμ. On a comparison of the colour curve of the sample to be tested with that of the typical colour curve of chlorophyll the presence of carotenoids at once becomes evident, and carotin and xanthophyll are identified separately by the positions of the band maxima in the region 500μμ to 430μμ.

Carotenoids in as low a concentration as 0.05 % are detected by the new method described.

Keywords

Alcohol Methyl Chlorophyll Carotenoid Typical Colour 

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References

  1. 1.
    Jörgensen, I. andKidd, F., 1917, Some photochemical experiments with pure chlorophyll and their bearing on carbon assimilation. Proc. Roy. Soc. B. 89, 1.Google Scholar
  2. 2.
    Singh, B. N. andAnantha Rao, N. K., 1937, Colour curves and purity tests in physiological researches. Jour. Gen. Physiol. (in course of publication).Google Scholar
  3. 3.
    -, 1937, A newPhotoelectric Photometer for chemical analysis. Plant Physiol. (in press).Google Scholar

Copyright information

© Verlag Von Gebrüder Borntraeger 1938

Authors and Affiliations

  • B. N. Singh
    • 1
  • N. K. Anantha Rao
    • 1
  1. 1.Plant Physiological Laboratories, Institute of Agricultural ResearchBenares Hindu UniversityIndia

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