Skip to main content

Tumorigenicity, invasion, and metastasis of human gastric cancer in nude mice

Journal of Cancer Research and Clinical Oncology volume 117, pages 533–538 (1991)Cite this article

Summary

Tumors derived from 105 patients with gastric cancer were subcutaneously heterotransplanted into nude mice in order to study their tumorigenicity and malignant behavior. Of the 105 gastric cancers, 45 were successfully transplanted (a 42.9% tumorigenesis rate). The tumorigenesis rate of Borrmann type 1 and 2 cancers (77.8%) was significantly higher than that of type 3 and 4 cancers (34.6%). Also, the tumorigenesis rate of differentiated carcinoma (57.1 %) was significantly higher than that of undifferentiated carcinoma (30.9%). Spontaneous metastases from the subcutaneous tumors were observed in 5 of the 37 established tumor lines (13.5%), and macroscopic pulmonary metastases were common with one tumor line (SCK-29). Although most of the subcutaneous gastric cancers showed local expansion without distant metastasis, the same tumor cells implanted into the peritoneal cavity exhibited invasive growth and/or metastasis. Thus, the expression of a metastatic pheno-type by human gastric cancer was influenced by the host microenvironment. The SCK-29 tumor line with its high metastatic potential may be useful for studies on the mechanism of blood-borne metastasis.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Abbreviations

pap:

papillary adenocarcinoma

tub1 :

well-differentiated adenocarcinoma

tub2 :

moderately differentiated adenocarcinoma

por:

poorly differentiated adenocarcinoma

sig:

signet-ring cell adenocarcinoma

muc:

mucinus adenocarcinoma

giant:

giant cell adenocarcinoma

ud:

undifferentiated adenocarcinoma

ade:

ascitic adenocarcinoma

References

  • Fogh J, Fogh JM, Orfeo T (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59:221–226

    Google Scholar 

  • Giavazzi R, Campbell DE, Jessup JM, Cleary K, Fidler IJ (1986) Metastatic behavior of tumor cells isolated from primary and metastatic human colorectal carcinomas implanted into different site in nude mice. Cancer Res 46:1928–1933

    Google Scholar 

  • Hata J, Ueyama Y, Tamaoki N, Furukawa T, Morita K (1978) Human neuroblastoma serially transplanted in nude mice and metastases. Cancer 42:468–473

    Google Scholar 

  • Hirohashi S, Shimosato Y, Kameya T, Nagai K, Tsunematsu R (1977) Hormone dependency of a serially transplantable human breast cancer (Br-10) in nude mice. Cancer Res 37:3184–3189

    Google Scholar 

  • Katano M, Saxton RE, Cochran AJ, Irie RF (1984) Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice. J Cancer Res Clin Oncol 108:197–203

    Google Scholar 

  • Kerbel RS, Man MS, Dexter D (1984) A model of human cancer metastasis: extensive spontaneous and artificial metastasis of a pigmented melanoma and derived variant sublines in nude mice. J Natl Cancer Inst 72:93–108

    Google Scholar 

  • Kubota T, Shimosato Y, Nagai K (1978) Experimental chemotherapy of carcinoma of the human stomach and colon serially transplanted in nude mice. Jpn J Cancer Res 69:299–309

    Google Scholar 

  • Kuroki M (1984) A new human osteosarcoma strain (Os-Beppu) with a high rate of pulmonary metastasis in nude mice. Jpn J Clin Oncol 14:49–56

    Google Scholar 

  • Kyriazis AP, Kyriazis AA, McCombs WB, Kereiakes JA (1981) Biological behavior of human malignant tumors grown in the nude mouse. Cancer Res 41:3995–4000

    Google Scholar 

  • Naito S, Eschenbach AC, Fidler IJ (1987) Different growth pattern and biologic behavior of human renal cell carcinoma implanted into different organs of nude mice. J Natl Cancer Inst 78:377–385

    Google Scholar 

  • Naomoto Y, Kondo H, Tanaka N, Orita K (1987) Novel experimental models of human cancer metastasis in nude mice: lung metastasis, intraabdominal carcinomatosis with ascites, and liver metastasis. J Cancer Res Clin Oncol 113:544–549

    Google Scholar 

  • Otake Y (1980) Establishment of a metastasizing human breast cancer cell line in nude mice. Fukushima J Med Sci 27:53–62

    Google Scholar 

  • Ozzello L, Sordat M (1980) Behavior of tumors produced by transplantation of human mammary cell lines in athymic nude mice. Eur J Cancer 16:553–559

    Google Scholar 

  • Rygaard J, Povlsen CO (1969) Heterotransplantation of a human malignant tumor to nude mice. Acta Pathol Microbiol Scand 77:758–760

    Google Scholar 

  • Sattler FR, Paolucci S, Kreider JW, Ladda RL (1982) A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice. Eur J Cancer Oncol 18:381–389

    Google Scholar 

  • Schmidt M, Deshner EE (1977) Gastrointestinal cancer studies in the human to nude mouse heterotransplantation system. Gastroenterology 72:829

    Google Scholar 

  • Sharkey FE, Fogh H (1979) Metastasis of human tumors in athymic nude mice. Int J Cancer 24:733–738

    Google Scholar 

  • Shimosato Y, Kameya T, Nagai K, Hirohashi S, Koide T, Hayashi H, Namura T (1976) Transplantation of human tumors in nude mice. J Natl Cancer Inst 56:1251–1255

    Google Scholar 

  • Stragand JJ, Barlogie B, White RA, Drewinko B (1981) Biological properties of the human colonic adenocarcinoma cell lines SW 620 grown as a xenograft in the athymic mouse. Cancer Res 41:3364–3369

    Google Scholar 

  • Trope C, Wennerberg J, Johansson S, Unsgard B (1982) Metastasis of a cultured human bladder carcinoma cell line transplanted into nude athymic mice. Neoplasma 29:421–426

    Google Scholar 

  • Ueyama Y, Morita K, Ochiai C, Ohsawa N, Hata J, Tamaoki N (1978) Xenotransplantation of a human meningioma and its lung metastasis in nude mice. Br J Cancer 37:644–647

    Google Scholar 

  • Ware JL, Paulson DF, Mickey GH, Webb KS (1982) Spontaneous metastasis of cells of the human prostate carcinoma cell line PC-3 in athymic nude mice. J Urol 128:1064–1067

    Google Scholar 

  • Yamashita T (1988) Manifestation of metastatic potential in human gastric cancer implanted into the stomach wall of nude mice. Jpn J Cancer Res 79:945–951

    Google Scholar 

Download references

Author information

Affiliations

  1. First Department of Surgery, Kagoshima University, School of Medicine, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan

    Sonshin Takao, Hisaaki Shimazu, Shigeho Maenohara, Schuichi Hokita & Takashi Aikou

Authors
  1. Sonshin Takao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hisaaki Shimazu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shigeho Maenohara
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Schuichi Hokita
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takashi Aikou
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This study was supported in part by a grant-in aid for cancer research (B.no. 63480309) from the Ministry of Education, Science and Culture, Japan

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Takao, S., Shimazu, H., Maenohara, S. et al. Tumorigenicity, invasion, and metastasis of human gastric cancer in nude mice. J Cancer Res Clin Oncol 117, 533–538 (1991). https://doi.org/10.1007/BF01613284

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01613284

Key words

  • Gastric cancer
  • Nude mouse
  • Tumorigenicity
  • Invasion
  • Metastasis