Summary
Tumors derived from 105 patients with gastric cancer were subcutaneously heterotransplanted into nude mice in order to study their tumorigenicity and malignant behavior. Of the 105 gastric cancers, 45 were successfully transplanted (a 42.9% tumorigenesis rate). The tumorigenesis rate of Borrmann type 1 and 2 cancers (77.8%) was significantly higher than that of type 3 and 4 cancers (34.6%). Also, the tumorigenesis rate of differentiated carcinoma (57.1 %) was significantly higher than that of undifferentiated carcinoma (30.9%). Spontaneous metastases from the subcutaneous tumors were observed in 5 of the 37 established tumor lines (13.5%), and macroscopic pulmonary metastases were common with one tumor line (SCK-29). Although most of the subcutaneous gastric cancers showed local expansion without distant metastasis, the same tumor cells implanted into the peritoneal cavity exhibited invasive growth and/or metastasis. Thus, the expression of a metastatic pheno-type by human gastric cancer was influenced by the host microenvironment. The SCK-29 tumor line with its high metastatic potential may be useful for studies on the mechanism of blood-borne metastasis.
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Abbreviations
- pap:
-
papillary adenocarcinoma
- tub1 :
-
well-differentiated adenocarcinoma
- tub2 :
-
moderately differentiated adenocarcinoma
- por:
-
poorly differentiated adenocarcinoma
- sig:
-
signet-ring cell adenocarcinoma
- muc:
-
mucinus adenocarcinoma
- giant:
-
giant cell adenocarcinoma
- ud:
-
undifferentiated adenocarcinoma
- ade:
-
ascitic adenocarcinoma
References
Fogh J, Fogh JM, Orfeo T (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59:221–226
Giavazzi R, Campbell DE, Jessup JM, Cleary K, Fidler IJ (1986) Metastatic behavior of tumor cells isolated from primary and metastatic human colorectal carcinomas implanted into different site in nude mice. Cancer Res 46:1928–1933
Hata J, Ueyama Y, Tamaoki N, Furukawa T, Morita K (1978) Human neuroblastoma serially transplanted in nude mice and metastases. Cancer 42:468–473
Hirohashi S, Shimosato Y, Kameya T, Nagai K, Tsunematsu R (1977) Hormone dependency of a serially transplantable human breast cancer (Br-10) in nude mice. Cancer Res 37:3184–3189
Katano M, Saxton RE, Cochran AJ, Irie RF (1984) Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice. J Cancer Res Clin Oncol 108:197–203
Kerbel RS, Man MS, Dexter D (1984) A model of human cancer metastasis: extensive spontaneous and artificial metastasis of a pigmented melanoma and derived variant sublines in nude mice. J Natl Cancer Inst 72:93–108
Kubota T, Shimosato Y, Nagai K (1978) Experimental chemotherapy of carcinoma of the human stomach and colon serially transplanted in nude mice. Jpn J Cancer Res 69:299–309
Kuroki M (1984) A new human osteosarcoma strain (Os-Beppu) with a high rate of pulmonary metastasis in nude mice. Jpn J Clin Oncol 14:49–56
Kyriazis AP, Kyriazis AA, McCombs WB, Kereiakes JA (1981) Biological behavior of human malignant tumors grown in the nude mouse. Cancer Res 41:3995–4000
Naito S, Eschenbach AC, Fidler IJ (1987) Different growth pattern and biologic behavior of human renal cell carcinoma implanted into different organs of nude mice. J Natl Cancer Inst 78:377–385
Naomoto Y, Kondo H, Tanaka N, Orita K (1987) Novel experimental models of human cancer metastasis in nude mice: lung metastasis, intraabdominal carcinomatosis with ascites, and liver metastasis. J Cancer Res Clin Oncol 113:544–549
Otake Y (1980) Establishment of a metastasizing human breast cancer cell line in nude mice. Fukushima J Med Sci 27:53–62
Ozzello L, Sordat M (1980) Behavior of tumors produced by transplantation of human mammary cell lines in athymic nude mice. Eur J Cancer 16:553–559
Rygaard J, Povlsen CO (1969) Heterotransplantation of a human malignant tumor to nude mice. Acta Pathol Microbiol Scand 77:758–760
Sattler FR, Paolucci S, Kreider JW, Ladda RL (1982) A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice. Eur J Cancer Oncol 18:381–389
Schmidt M, Deshner EE (1977) Gastrointestinal cancer studies in the human to nude mouse heterotransplantation system. Gastroenterology 72:829
Sharkey FE, Fogh H (1979) Metastasis of human tumors in athymic nude mice. Int J Cancer 24:733–738
Shimosato Y, Kameya T, Nagai K, Hirohashi S, Koide T, Hayashi H, Namura T (1976) Transplantation of human tumors in nude mice. J Natl Cancer Inst 56:1251–1255
Stragand JJ, Barlogie B, White RA, Drewinko B (1981) Biological properties of the human colonic adenocarcinoma cell lines SW 620 grown as a xenograft in the athymic mouse. Cancer Res 41:3364–3369
Trope C, Wennerberg J, Johansson S, Unsgard B (1982) Metastasis of a cultured human bladder carcinoma cell line transplanted into nude athymic mice. Neoplasma 29:421–426
Ueyama Y, Morita K, Ochiai C, Ohsawa N, Hata J, Tamaoki N (1978) Xenotransplantation of a human meningioma and its lung metastasis in nude mice. Br J Cancer 37:644–647
Ware JL, Paulson DF, Mickey GH, Webb KS (1982) Spontaneous metastasis of cells of the human prostate carcinoma cell line PC-3 in athymic nude mice. J Urol 128:1064–1067
Yamashita T (1988) Manifestation of metastatic potential in human gastric cancer implanted into the stomach wall of nude mice. Jpn J Cancer Res 79:945–951
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This study was supported in part by a grant-in aid for cancer research (B.no. 63480309) from the Ministry of Education, Science and Culture, Japan
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Takao, S., Shimazu, H., Maenohara, S. et al. Tumorigenicity, invasion, and metastasis of human gastric cancer in nude mice. J Cancer Res Clin Oncol 117, 533–538 (1991). https://doi.org/10.1007/BF01613284
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DOI: https://doi.org/10.1007/BF01613284