Summary
Immunological characterization of tumour infiltrating lymphocytes (TIL) by immunohistological techniques was carried out in 20 cases of stage I seminoma. Routine pathological examination of these surgical specimens showed typical seminoma in 20 cases. Eighteen cases showed obvious TIL and immunohistological staining on frozen specimens was performed in 12. TIL in seminomas were predominantly T-cells but B-cells were also identified. T-cells were distributed diffusely with predominance of the CD 8+ phenotype judged semiquantitatively. In contrast to the distribution of T-cells, B-cells tended to accumulate and occasionally formed lymphoid follicles. In such follicles the phenotypic pattern of B-cell antigens was comparable with secondary lymphoid follicles in lymphoid organs. There is an immunologically complex response to seminoma by the host with a predominant infiltration of cytotoxic/ suppressor T-cells and functional maturation of B-cells.
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References
Bell DA, Flotte TJ, Bhan AK (1987) Immunohistochemical characterization of seminoma and inflammatory cell infiltrate. Hum Pathol 18:511–520
Gemmell E, Strutton GM, Seymour GJ, Walsh MD, Lavin MF, Gardiner RA (1988) An immunohistopathological characterisation of mixed non-seminomatous germ cell tumor. Urol Res 16:13–17
Häyry P, Tötterman TH (1978) Cytological and functional analysis of inflammatory infiltrates in human malignant tumors. I. Composition of the inflammatory infiltrates. Eur J Immunol 8:866–871
Ioachim HL (1976) The stromal reaction of tumors: an expression of immune surveillance. J Natl Cancer Inst 57:465–475
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA (1981) Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med 153:310–323
Mostofi FK, Sesternhenn I (1976) Lymphocytic infiltration in relationship to urologic tumors. NCI Monogr 49:133–141
Schütte B, Holstein AF, Schirren C (1988) Macrophages lysing seminoma cells in patients with carcinoma-in-situ (CIS) of the testis. Andrologia 20:295–303
Shimamura K, Osamura Y, Hata J, Tamaoki N, Watanabe K, Kubota M, Nakazaki H, Tajima T, Mitomi T, Yoneyama K, Hayashi T (1977) Gastric carcinoma with lymphoid stroma. Clinicopathological studies of four cases. Tokai Exp Clin Med 2:229–234
Shimokawara I, Imamura M, Kikuchi K (1982) Identification of lymphocyte subpopulations in human breast cancer tissue and its significance. An immunoperoxidase study with antihuman T- and B-cell sera. Cancer 49:1456–1464
Takami T, Ishii Y, Yuasa H, Kikuchi K (1985) Three distinct antigen systems on human B cell subpopulations as defined by monoclonal antibodies. J Immunol 134:828–834
Thackray AC, Crane WAJ (1976) Seminoma. In: Pugh RCB (ed) Pathology of the testis. Blackwell, Oxford, pp 164–198
Uede T, Kohda H, Ibayashi Y, Osawa H, Diamantstein T, Kikuchi K (1985) Functional analysis of mononuclear cells infiltrating into tumors. II. Differential ability of mononuclear cells obtained from various tissues to produce helper factors that are involved in the generation of cytotoxic cells. J Immunol 135:3243–3251
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Nakanoma, T., Nakamura, K., Deguchi, N. et al. Immunohistological analysis of tumour infiltrating lymphocytes in seminoma using monoclonal antibodies. Vichows Archiv A Pathol Anat 421, 409–413 (1992). https://doi.org/10.1007/BF01606913
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DOI: https://doi.org/10.1007/BF01606913