Skip to main content
SpringerLink
Log in

Haemopoiesis in human fetal and embryonic liver

Immunohistochemical determination in B5-fixed paraffin-embedded tissues

  • Published:
Virchows Archiv A Aims and scope Submit manuscript

Summary

Paraffin embedded tissue reactive monoclonal antibodies were used to study human embryonal and fetal haemopoiesis, combining optimal morphology with immunohistological determination of haemopoietic cell subtypes and their microenvironment. Seven embryonal and twelve fetal liver specimens were studied, having been fixed in B5-fixative and embedded in paraffin. The different haemopoietic lineages each showed their own immunophenotype and distribution; intercellular and microenvironmental relationships were easily determined. Erythroid cells are reactive with VIE-G4, LN1, and MT1, sometimes partly surrounding a central macrophage. Myelomonocytic cells react with LCA, MT1, MB3, LN2, and anti-lysozyme, and from 14 weeks onwards with LN3. Lymphoid cells show LCA, MT1, MT2, MB1, MB2, MB3, and LN2 reactivity. In a few cases some scarce My10+ early progenitor cells were seen. An important finding is the extensive MT1-reactivity distributed over all haemopoietic lineages, and the demonstration of immature haemopoietic blast cells exclusively expressing the MT1 antigen. Further studies employing MT1 are necessary to delineate the extent of the distribution and the possible function of the antigen. Use of the MT1 mAb may contribute to the elucidation of the exact nature of the haemopoietic blast cells and their place in haemopoietic development.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bodger MP, Izaguirre CA, Blacklock HA, Hoffbrand AV (1983) Surface antigenic determinants on human pluripotent and unipotent hematopoietic progenitor cells. Blood 61(5): 1006–1010

    Google Scholar 

  • Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH (1984) Antigenic analysis of hematopoiesis: III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol 133(1): 157–165

    Google Scholar 

  • Dellagi K, Vainchenker W, Vinci G, Paulin D, Brouet JC (1983) Alteration of vimentin intermediate filament expression during differentiation of human hemopoietic cells. EMBO J 2(9): 1509–1514

    Google Scholar 

  • Emura I, Sekiya M, Ohnishi Y (1983) Four types of presumptive hemopoietic stem cells in the human fetal liver. Arch Histol Jap 46(5): 645–662

    Google Scholar 

  • Emura I, Sekiya M, Ohnishi Y (1984) Ultrastructural identification of the hemopoietic inductive microenvironment in the human embryonic liver. Arch Histol Jap 47(1): 95–112

    Google Scholar 

  • England MA (1983) A colour atlas of life before birth: Normal fetal development, Wolfe, London

    Google Scholar 

  • Enzan H, Takahashi H, Kawakami M, Yamashita S, Ohkita T (1978) Light and electronmicroscopic observations on hepatic hematopoiesis of human fetuses: I. Granulocytopoiesis in the hepatic mesenchymal tissue. Acta Path Jap 28(3): 411–426

    Google Scholar 

  • Epstein AL, Marder RJ, Winter JN, Fox RI (1984) Two new monoclonal antibodies (LN1, LN2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol 133(2): 1028–1036

    Google Scholar 

  • Fukuda T (1974) Fetal hemopoiesis: II. Electron-microscopic studies on human hepatic hemopoiesis. Virchows Arch [B] 16:249–270

    Google Scholar 

  • Gilmour JR (1941) Normal hemopoiesis in intra-uterine and fetal life. J Pathol Bacteriol 52:25–55

    Google Scholar 

  • Hofman FM, Danilovs J, Husmann L, Taylor CR (1984) Ontogeny of B cell markers in the human fetal liver. J Immunol 133(3):1197–1201

    Google Scholar 

  • Hokland P, Rosenthal P, Griffin JD, Nadler LM, Daley JF, Hokland M, Schlossman SF, Ritz J (1983) Purification and characterization of fetal hematopoietic cells that express the common acute lymphoblastic leukemia antigen (CALLA). J Exp Med 157:114–129

    Google Scholar 

  • Kamps WA, Cooper MD (1982) Microenvironmental studies of pre-B and B cell development in human and mouse fetuses. J Immunol 129(2):526–531

    Google Scholar 

  • Kamps WA, Timens W, de Boer TGJ, Spanjer HH, Poppema S (1989) In situ study of hemopoiesis in human fetal liver. Scand J Immunol 30:399–408

    Google Scholar 

  • Kelemen E, Calvo W, Fliedner TM (1979) Atlas of human hemopoietic development. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Liszka K, Majdic O, Bettelheim P, Knapp W (1983) Glycophorin A expression in malignant hematopoiesis. Am J Hematol 15:219–226

    Google Scholar 

  • Lu L, Walker D, Broxmeyer HE, Hoffman R, Hu W, Walker E (1987) Characterization of adult marrow hematopoietic progenitors highly enriched by two color cell sorting with My10 and major histocompatibility class II monoclonal antibodies. J Immunol 139:1823–1829

    Google Scholar 

  • Magyarlaki T, Kelenyi G (1988) Use of monoclonal antibodies in formol-paraffin sections. Virchows Arch [A] 412:487–492

    Google Scholar 

  • Migliaccio G, Migliaccio AR, Petti S, Mavilio F, Russo G, Lazarro D, Testa U, Marinucci M, Peschle C (1986) Human embryonic hemopoiesis: Kinetics of progenitors and precursors underlying the yolk sac to liver transition. J Clin Invest 78:51–60

    Google Scholar 

  • Moore KL (1982) The Developing Human. Saunders, Philadelphia

    Google Scholar 

  • Natali PG, Segatto O, Ferrone S, Tosi R, Corte G (1987) Differential tissue distribution and ontogeny of DC-1 and HLA-DR antigens. Immunogenetics 19:109–116

    Google Scholar 

  • O'Rahilly R (1979) Early human development and the chief sources of information on staged human embryos. Europ J Obstet Gynec Reprod Biol 9/4:273–280

    Google Scholar 

  • Peschle C, Migliaccio G, Lazarro D, Petti S, Mancini G, Care A, Russo G, Mastroberardino G, Migliaccio AR, Testa U (1984) Hemopoietic development in human embryos. Blood Cells 10:427–441

    Google Scholar 

  • Poppema S, Hollema H (1987) Monoclonal antibodies in lymphoma diagnosis and classification. In: Ruiter DJ, Fleuren GJ, Warnaar SO (eds) Application of monoclonal antibodies in tumor pathology. Martinus Nijhoff, Dordrecht, The Netherlands, pp 117–130

    Google Scholar 

  • Poppema S, Hollema H, Visser L, Vos H (1987) Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leucocyte subsets in paraffin-embedded tissue sections. Am J Pathol 127:418–429

    Google Scholar 

  • Quaranta V, Majdic O, Stingl G, Liszka K, Honigsman H, Knapp W (1984) A human Ia cytoplasmic determinant located on multiple forms of invariant chain (gamma, gamma 2, gamma 3). J Immunol 132(4):1900–1905

    Google Scholar 

  • Rosenthal P, Rimm IJ, Umiel T, Griffin JD, Osathanondh R, Schlossman SF, Nadler LM (1983) Ontogeny of human hematopoietic cells: analysis utilizing monoclonal antibodies. J Immunol 131:232–237

    Google Scholar 

  • Sieff C, Bicknell D, Caine G, Robinson J, Lam G, Greaves MF (1982) Changes in cell surface antigen expression during hemopoietic differentiation. Blood 60(3): 703–713

    Google Scholar 

  • Timens W, Rozeboom-Uiterwijk T, Poppema S (1988) Immunoarchitecture of human fetal lymphoid tissues. Virchows Arch [A] 413:563–571

    Google Scholar 

  • Van der Valk P, Mullink H, Huijgens PC, Tadema TM, Vos W, Meijer CJLM (1989) Immunohistochemistry in bone marrow diagnosis. Value of a panel of monoclonal antibodies on routinely processed bone marrow biopsies. Am J Surg Pathol 13:97–106

    Google Scholar 

  • Warnke RA, Gatter KC, Phil D, Falini B, Hildreth P, Woolston RE, Pulford K, Cordell JL, Cohen B, De Wolf-Peeters C, Mason DY (1983) Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 309:1275–1281

    Google Scholar 

Download references

Author information

Author notes

  1. Sibrand Poppema FRCP(C) (Professor of Pathology; Director of Pathology and Lab. Medicine)

    Present address: Cross Cancer Institute, T6G 1Z2, Edmonton, Alberta, Canada

Authors and Affiliations

  1. Department of Pediatric Oncology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands

    Willem A. Kamps

  2. Departments of Pathology, University of Groningen, Oostersingel 63, 9713, EZ Groningen, The Netherlands

    Wim Timens, Willem A. Kamps, Thea Rozeboom-Uiterwijk & Sibrand Poppema FRCP(C) (Professor of Pathology; Director of Pathology and Lab. Medicine)

Authors
  1. Wim Timens
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Willem A. Kamps
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thea Rozeboom-Uiterwijk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sibrand Poppema FRCP(C)
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Timens, W., Kamps, W.A., Rozeboom-Uiterwijk, T. et al. Haemopoiesis in human fetal and embryonic liver. Vichows Archiv A Pathol Anat 416, 429–436 (1990). https://doi.org/10.1007/BF01605149

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01605149

Key words

Search

Navigation