Summary
An in situ hybridization assay with digoxigenin-labelled probes was used to detect the presence of human papillomavirus (HPV) sequences in ten related Venezuelan patients with the diagnosis of focal epithelial hyperplasia. The samples displayed HPV sequences in all cases. Further restriction analysis in four of the patients suggested the presence of HPV-13 in oral lesions.
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References
Archard HO, Heck JW, Stanley HR (1965) Focal epithelial hyperplasia: an unusual oral mucosal lesion found in Indian children. Oral Surg 20:201–212
Beaudenon S, Praetorius F, Kremsdorf D, Lutzner M, Worsaae N, Pehaw-Arnaudet G, Orth G (1987) A new type of human papillomavirus associated with oral focal epithelial hyperplasia. Invest Dermatol 88:130–135
Denhardt DT (1966) A membrane filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641
De Villiers EM, Neumann C, Le JY, Weiduer H, zur Hausen H (1986) Infection of the oral mucosa with defined types of human papillomaviruses. Med Microbiol Immunol (Berl) 174:287–294
Estrada L (1956) Aporte al estudio odontologico de los Indios Ratios. Heraldo Dental 2:5
Gomez A, Calle C, Arala G, Pindborg JT (1969) Focal epithelial hyperplasia in a half-breed familial of Colombians. J Am Dent Assoc 79:663–667
Goodfellow A, Calvert H (1979) Focal epithelial hyperplasia of the oral mucosa. Br J Dermatol 101:341–344
Hanks CT, Fischman SL, Guzman MN de (1972) Focal epithelial hyperplasia. A light and electron microscopic study of one case. Oral Surg 33:934–943
Henke RP, Milde-Langosch K, Loning T, Koppang HS (1987) Human papillomavirus type 13 and focal epithelial hyperplasia of the oral mucosa: DNA hybridization on paraffin embedded specimens. Virchows Arch [A] 411:193–198
Hernandez-Jauregui P, Eriksson A, Tamayo Perez R, Pettersson U, Moreno-Lopez J (1987) Human papillomavirus type 13 DNA in focal epithelial hyperplasia among Mexicans. Arch Virol 93:131–137
Kuffer R, Perol Y (1976) Hyperplasie epitheliale focale. Premier cas français. Mise en évidence d'un virus papova en microscopie électronique. Rev Stomatol 77:318–321
Kuhlwein A, Nasemann T, Janner M, Schaeg G, Reinel D (1982) Nachweis von Papillomaviren bei fokaler epithelialer Hyperplasie Heck und die Differentialdiagnose zum weiben Schleimhautnävus. Hautarzt 32:617–621
Lang E, Zabel M, Ikemberg H (1984) Fokale epitheliale Hyperplasie (Morbus Heck), Dtsch Med Wochenschr 109:1763–1766
Maniatis T, Fritsh EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
March CJ (1881) Multiple papillary tumors of labial, buccal and glossal mucous membrane. Dent Cosmos 23:165
Petzoldt D, Dennin R (1980) Isolierung virusartiger Partikel bei fokaler epithelialer Hyperplasie Heck. Hautarzt 31:35–36
Petzoldt D, Pfister H (1980) HPV1 DNA in lesions of focal epithelial hyperplasia Heck. Arch Dermatol Res 268:313–314
Pfister H, Hettich I, Runne U, Gissmann L, Chilf GN (1983) Characterization of human papillomavirus type 13 from focal epithelial hyperplasia Heck lesions. J Virol 47:363–366
Pilgard G (1984) Focal epithelial hyperplasia. Oral Surg 57:540–543
Praetorius-Clausen F, Willis JM (1971) Papova virus-like particles in focal epithelial hyperplasia. Scand J Dent Res 79:362–365
Praetorius F, Praetorius-Clausen P, Mogeltoft M (1985) Immuno-histochemical evidence of papillomavirus antigen in focal epithelial hyperplasia. Tandlaegeblaadet 89:585–589
Reyes DG (1962) Verruga de la cavidad oral. Riv Colegic Med (Guatemala) 13:223
Sattgast JE, Limmer BL (1976) Focal epithelial hyperplasia: a case report with light and electron microscopic findings. J Assoc Mil Dermatol 2:41–44
Soneira A, Fonseca N (1964) Sobre una lesion de la mucosa oral en los niños de la Mision los Angeles del Tokuko. Venezuela Odont 29:109
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Syrjanen S, Syrjanen K, Ikenberg H, Gissmann L, Lamberg M (1984) A human papillomavirus closely related to HPV 13 found in a focal epithelial hyperplasia lesion (Heck disease). Arch Dermatol Res 276:199–200
Van Wyk CW, Staz J, Farman AG (1977) Focal epithelial hyperplasia in a group of South Africans: its ultrastructural features. J Oral Pathol 6:14–24
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Premoli-de-Percoco, G., Galindo, I. & Ramirez, J.L. In situ hybridization with digoxigenin-labelled DNA probes for the detection of human papillomavirus-induced focal epithelial hyperplasia among Venezuelans. Vichows Archiv A Pathol Anat 420, 295–300 (1992). https://doi.org/10.1007/BF01600207
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DOI: https://doi.org/10.1007/BF01600207