Summary
We describe a breast cancer with ectopic production of amylase, found in the patient's serum, urine and in the tumour. Clinically, serum amylase levels reflected both the progression of the disease and regression induced by various therapies. Using agarose gel electrophoresis and a wheat protein inhibitor assay, the predominant serum amylase appeared to be identical to pancreatic-type isoenzyme. However, the action mode analysis using a new fluorogenic substrate revealed that the serum contained non-salivary, non-pancreatic amylase. The tumour had microscopic features of invasive ductal carcinoma with some argyrophilic differentiation. The component cells stained positively for amylase, and ultrastructurally numerous secretory granules were seen.
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References
Ceska M, Birath K, Brown B (1969) A new and rapid method for the clinical determination ofα-amylase activities in human serum and urine: optimal conditions. Clin Chim Acta 26:437–444
Ende N (1960) Studies of amylase activity in pleural effusions and ascites. Cancer 13:283–287
Hata H, Matsuzaki H, Tanaka K, Nomura H, Kagimoto T, Takeya M, Yamane N, Takatsuki K (1988) Ectopic production of salivary-type amylase by a IgA-λ-type multiple myeloma. Cancer 62:1511–1515
Hodes ME, Sisk CJ, Kam RC, Ehrlich CE, Lehrner LM, Roth LM, Morley DJ, Merritt AD (1985) An amylase-producing serous cystadenocarcinoma of the ovary. Oncology 42:242–247
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques; a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Matsuyama M, Inoue T, Ariyoshi Y, Doi M, Suchi T, Sato T, Tashiro K, Chihara T (1979) Argyrophil cell carcinoma of the uterine cervix with ectopic production of ACTH,β-MSH, serotonin, histamine and amylase. Cancer 44:1813–1823
Nomura H, Tokumitsu S, Takeuchi T (1980) Ultrastructural, cytochemical, and biochemical characterization of alpha-amylase produced by human gastric cancer cells in vitro. J Natl Cancer Inst 64:1015–1024
Norwood SH, Torma MJ, Fontenelle LJ (1981) Hyperamylasemia due to poorly differentiated adenosquamous carcinoma of the ovary. Arch Surg 116:225–226
Okabe H, Uji Y, Netsu K, Noma A (1984) Automated measurement of amylase isoenzymes with 4-nitrophenylmaltoheptaoside as substrate and use of a selective amylase inhibitor. Clin Chem 30:1219–1222
Omichi K, Ikenaka T (1984) Differential assay of human pancreatic and salivaryα-amylases in serum using a new fluorogenic substrate. Clin Chim Acta 138:197–203
Shimamura J, Fridhandler L, Berk JE (1976) Unusual isoamylase in cancer-associated hyperamylasemia. Cancer 38:2121–2126
Shiosaki K, Takata K, Omichi K, Tomita N, Horii A, Ogawa M, Matsubara K (1990) Identification of a novelα-amylase by expression of a newly cloned human amy 3 cDNA in yeast. Gene 89:253–258
Takeuchi T, Fujiki H, Kameya T (1981) Characterization of amylases produced by tumors. Clin Chem 27:556–559
Tomita N, Matsuura N, Horii A, Emi M, Nishide T, Ogawa M, Mori T, Doi O, Matsubara K (1988) Expression of aα-amylase in human lung cancers. Cancer Res 48:3292–3296
Tomita N, Horii A, Doi S, Yokouchi H, Shiosaki K, Higashiyama M, Matsuura N, Ogawa M, Mori T, Matsubara K (1989) A novel type of humanα-amylase produced in lung carcinoid tumor. Gene 76:11–18
Weiss MJ, Edmondson HA, Wertman M (1951) Elevated serum amylase associated with bronchogenic carcinoma. Am J Clin Pathol 21:1051–1061
Weitzel JN, Pooler PA, Mohammed R, Levitt MD, Eckfeldt JH (1988) A unique case of breast carcinoma producing pancreatictype isoamylase. Gastroenterology 94:519–520
Yoshida Y, Mori M, Sonoda T, Sakauchi F, Sugawara H, Suzaki A (1985) Ultrastructural, immunohistochemical and biochemical studies on amylase and ACTH producing lung cancer. Virchows Arch [A] 408:163–172
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Inaji, H., Koyama, H., Higashiyama, M. et al. Immunohistochemical, ultrastructural and biochemical studies of an amylase-producing breast carcinoma. Vichows Archiv A Pathol Anat 419, 29–33 (1991). https://doi.org/10.1007/BF01600149
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DOI: https://doi.org/10.1007/BF01600149